Impact of CMV Reactivation, Treatment Approaches, and Immune Reconstitution in a Nonmyeloablative Tolerance Induction Protocol in Cynomolgus Macaques.

Transplantation
Paula Alonso-GuallartAdam Griesemer

Abstract

Cytomegalovirus (CMV) infection is a serious complication in immunosuppressed patients, specifically transplant recipients. Here, we describe the development and use of an assay to monitor the incidence and treatment of CMV viremia in a Cynomolgus macaque model of bone marrow transplantation (BMT) for tolerance induction. We address the correlation between the course of viremia and immune reconstitution. Twenty-one animals received a nonmyeloablative conditioning regimen. Seven received cyclosporine A for 28 days and 14 received rapamycin. A CMV polymerase chain reaction assay was developed and run twice per week to monitor viremia. Nineteen recipients were CMV seropositive before BMT. Immune reconstitution was monitored through flow cytometry and CMV viremia was tracked via quantitative polymerase chain reaction. Recipients developed CMV viremia during the first month post-BMT. Two animals developed uncontrollable CMV disease. CMV reactivation occurred earlier in cyclosporine A-treated animals compared with those receiving rapamycin. Post-BMT, T-cell counts remained significantly lower compared with pretransplant levels until CMV reactivation, at which point they increased during the viremic phase and approached pretransplant ...Continue Reading

References

Mar 1, 1986·The Journal of Infectious Diseases·J D MeyersE D Thomas
Nov 1, 1982·Reviews of Infectious Diseases·J D MeyersE D Thomas
Oct 10, 2006·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Stephanie A S StarasMichael J Cannon
Jan 15, 2008·Medical Microbiology and Immunology·Colin Powers, Klaus Früh
Jan 22, 2008·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Fausto BaldantiGiuseppe Gerna
Jan 29, 2008·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Christophe Legendre, Manuel Pascual
Dec 17, 2008·Advances in Virus Research·Yujuan Yue, Peter A Barry
Feb 18, 2010·Blood·Avital L AmirMirjam H M Heemskerk
May 22, 2010·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Ognjenka NadazdinGilles Benichou
Dec 15, 2010·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Karl S PeggsMark W Lowdell
Aug 11, 2011·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·G GernaE Arbustini
Oct 14, 2011·Journal of Virology·Angie K MarshKelly S MacDonald
Aug 7, 2012·Clinical and Experimental Immunology·S H C HavenithI J M Ten Berge
May 23, 2013·G3 : Genes - Genomes - Genetics·Julie A KarlDavid H O'Connor
Dec 18, 2013·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Haruko SugiyamaMitsune Tanimoto
May 23, 2014·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Per LjungmanUNKNOWN Infectious Diseases Working Party of the European Group for Blood and Marrow Transplantation
Feb 5, 2015·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·N MacesicJ Sasadeusz
Apr 11, 2015·Clinical & Translational Immunology·Chien-Li Holmes-LiewRajiv Khanna
Jul 30, 2015·Clinics·Luiz Sergio AzevedoHeloisa Helena de Sousa Marques
Apr 6, 2016·BMC Genomics·Justen N Hoffman RussellKelly S MacDonald
Aug 9, 2016·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·H B ZhengL S Kean
Nov 4, 2016·Journal of Virology·Jenny W CheRaymond M Welsh
Dec 7, 2017·The New England Journal of Medicine·Francisco M MartyCyrus Badshah
Apr 1, 2018·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·José Luis PiñanaDavid Navarro
Feb 16, 2019·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Paula Alonso-GuallartRaimon Duran-Struuck

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Citations

Jul 30, 2019·Transplantation·Deepali Kumar, Nazia Selzner
Jul 28, 2020·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Nicholas L PomplunEva G Rakasz

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Methods Mentioned

BETA
PCR
Flow
FCS
Genotyping

Software Mentioned

Fluidigm Access
GraphPad Prism
FCS
QuantStudio Real - Time PCR
EasyMag
Primer3

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