Impact of commonly used transplant immunosuppressive drugs on human NK cell function is dependent upon stimulation condition

PloS One
Aislin C MeehanGlen P Westall

Abstract

Lung transplantation is a recognised treatment for patients with end stage pulmonary disease. Transplant recipients receive life-long administration of immunosuppressive drugs that target T cell mediated graft rejection. However little is known of the impact on NK cells, which have the potential to be alloreactive in response to HLA-mismatched ligands on the lung allograft and in doing so, may impact negatively on allograft survival. NK cells from 20 healthy controls were assessed in response to Cyclosporine A, Mycophenolic acid (MPA; active form of Mycophenolate mofetil) and Prednisolone at a range of concentrations. The impact of these clinically used immunosuppressive drugs on cytotoxicity (measured by CD107a expression), IFN-γ production and CFSE proliferation was assessed in response to various stimuli including MHC class-I negative cell lines, IL-2/IL-12 cytokines and PMA/Ionomycin. Treatment with MPA and Prednisolone revealed significantly reduced CD107a expression in response to cell line stimulation. In comparison, addition of MPA and Cyclosporine A displayed reduced CD107a expression and IFN-γ production following PMA/Ionomycin stimulation. Diminished proliferation was observed in response to treatment with each drug....Continue Reading

References

Feb 1, 1981·Transplantation·M IntronaA Mantovani
Apr 27, 1997·Transplantation·E PeterssonG Hedlund
Mar 4, 1999·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·B A Lipsky, C A Baker
Nov 8, 2001·Trends in Immunology·M A CooperM A Caligiuri
Dec 4, 2003·Expert Opinion on Pharmacotherapy·Titte R SrinivasHerwig Ulf Meier-Kriesche
Dec 9, 2003·Journal of Immunological Methods·Markus J BartenJan F Gummert
Dec 18, 2004·Journal of Immunological Methods·Galit AlterMarcus Altfeld
Jan 7, 2005·Seminars in Thoracic and Cardiovascular Surgery·James S Allan
Feb 19, 2005·Nature Reviews. Immunology·Peter Parham
Jun 4, 2005·Advances in Experimental Medicine and Biology·Wayne M Yokoyama
May 9, 2006·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Judith M MortonAllan R Glanville
Dec 22, 2007·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Susan StewartSamuel A Yousem
Jan 15, 2008·Transplantation·Lu-En WaiSheri M Krams
Feb 13, 2008·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·James E FildesColm T Leonard
Feb 16, 2008·American Journal of Reproductive Immunology : AJRI·Meen-Yau ThumAmolak Bansal
Apr 30, 2008·Journal of Autoimmunity·Ruth Y LanM Eric Gershwin
Sep 4, 2008·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Johanna M Kwakkel-van ErpHenny G Otten
Apr 24, 2009·Transplant International : Official Journal of the European Society for Organ Transplantation·Johann PratschkeKatja Kotsch
May 6, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Anne-Hélène PilletThierry Rose
Dec 19, 2009·Transplantation·Aislin C MeehanGlen P Westall
Apr 1, 2010·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·W van der Touw, J S Bromberg
Oct 5, 2010·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·D N EissensI Joosten
Oct 23, 2010·PloS One·Olivier MorteauChristopher A O'Callaghan
Mar 18, 2011·Journal of Innate Immunity·Joshua D Brandstadter, Yiping Yang
Jun 22, 2011·Journal of Immunological Methods·Hilary S Warren

❮ Previous
Next ❯

Citations

Nov 26, 2015·The Journal of Infectious Diseases·Claudia Stuehler, Nina Khanna
Dec 23, 2015·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·C HarmonD D Houlihan
Nov 7, 2015·Journal of Immunology Research·Priscila de Matos SilvaPatricia Ucelli Simioni
Oct 15, 2013·Experimental Gerontology·David J SnareJulia Kubanek
Feb 19, 2017·Journal of Autoimmunity·Gaetane NocturneXavier Mariette
Jan 10, 2018·Frontiers in Immunology·Emilie DugastSophie Brouard
Feb 8, 2018·Journal of Immunology Research·Gisella Puga YungJörg D Seebach
Jun 28, 2017·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·M D ParkesL G Hidalgo
May 6, 2019·Experimental and Clinical Transplantation : Official Journal of the Middle East Society for Organ Transplantation·Azita Talebzadeh BonakdarAli Akbar Amirzargar
Nov 22, 2018·Transplantation·Christopher M HarpurLucy C Sullivan
Nov 2, 2018·Thorax·Daniel R CalabreseJohn R Greenland
Aug 10, 2019·Frontiers in Immunology·Samuel Cern Cher ChiangRebecca A Marsh
Feb 29, 2020·Trends in Immunology·Jasper IskeStefan G Tullius
Mar 2, 2021·Frontiers in Immunology·Christian BinderDavid Berglund
Jul 25, 2021·Journal of the American Society of Nephrology : JASN·Jasper CallemeynMaarten Naesens

❮ Previous
Next ❯

Methods Mentioned

BETA
bronchoalveolar
biopsy
FCS
flow cytometry
flow cytometry gating
PMA

Software Mentioned

FlowJo
GraphPad Prism
GraphPad

Related Concepts

Related Feeds

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.

Cancer Biology: Molecular Imaging

Molecular imaging enables noninvasive imaging of key molecules that are crucial to tumor biology. Discover the latest research in molecular imaging in cancer biology in this feed.

Cell Adhesion Molecules in the Brain

Cell adhesion molecules found on cell surface help cells bind with other cells or the extracellular matrix to maintain structure and function. Here is the latest research on their role in the brain.