Impact of conduit geometry on the performance of typical particulate microchip packings

Analytical Chemistry
Stephanie JungUlrich Tallarek

Abstract

This work investigates the impact of conduit geometry on the chromatographic performance of typical particulate microchip packings. For this purpose, high-performance liquid chromatography (HPLC)/UV-microchips with separation channels of quadratic, trapezoidal, or Gaussian cross section were fabricated by direct laser ablation and lamination of multiple polyimide layers and then slurry-packed with either 3 or 5 microm spherical porous C8-silica particles under optimized packing conditions. Experimentally determined plate height curves for the empty microchannels are compared with dispersion coefficients from theoretical calculations. Packing densities and plate height curves for the various microchip packings are presented and conclusively explained. The 3 microm packings display a high packing density irrespective of their conduit geometries, and their performance reflects the dispersion behavior of the empty channels. Dispersion in 5 microm packings correlates with the achieved packing densities, which are limited by the number and accessibility of corners in a given conduit shape.

References

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Citations

Jun 11, 2011·Se pu = Chinese journal of chromatography·Yafeng GuanChunfeng Duan
Dec 30, 2015·Chromatography·James P Grinias, Robert T Kennedy
Jul 7, 2010·Electrophoresis·Karine Faure
May 18, 2011·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·Siarhei KhirevichUlrich Tallarek
Mar 23, 2011·Journal of Separation Science·Stephanie JungUlrich Tallarek
Jun 8, 2011·Journal of Separation Science·Hui WangYukui Zhang
Dec 4, 2019·Mass Spectrometry Reviews·Przemyslaw MielczarekMarek Smoluch
Feb 22, 2021·Journal of Chromatography. a·Deyber Arley Vargas MedinaFernando Mauro Lanças
Feb 22, 2011·Journal of Chromatography. a·Stephanie JungUlrich Tallarek
Nov 20, 2012·Analytical Chemistry·Gert Desmet, Sebastiaan Eeltink

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