Impact of congenital cytomegalovirus infection on transcriptomes from archived dried blood spots in relation to long-term clinical outcome

PloS One
Roberta RovitoAnn C T M Vossen

Abstract

Congenital Cytomegalovirus infection (cCMV) is the leading infection in determining permanent long-term impairments (LTI), and its pathogenesis is largely unknown due to the complex interplay between viral, maternal, placental, and child factors. The cellular activity, considered to be the result of the response to exogenous and endogenous factors, is captured by the determination of gene expression profiles. In this study, we determined whole blood transcriptomes in relation to cCMV, CMV viral load and LTI development at 6 years of age by using RNA isolated from neonatal dried blood spots (DBS) stored at room temperature for 8 years. As DBS were assumed to mainly reflect the neonatal immune system, particular attention was given to the immune pathways using the global test. Additionally, differential expression of individual genes was performed using the voom/limma function packages. We demonstrated feasibility of RNA sequencing from archived neonatal DBS of children with cCMV, and non-infected controls, in relation to LTI and CMV viral load. Despite the lack of statistical power to detect individual genes differences, pathway analysis suggested the involvement of innate immune response with higher CMV viral loads, and of anti...Continue Reading

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Citations

Jul 17, 2020·Nature Communications·Christopher P OuelletteAsuncion Mejias
Jul 8, 2021·Current Opinion in Infectious Diseases·Asuncion MejiasOctavio Ramilo

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Methods Mentioned

BETA
PCR
Assay
RNA-seq

Software Mentioned

count
limma
FastQC
QuickGo browser
Ensembl
BIOPET Gentrap
R
Cutadapt
GSNAP
R package LIMMA

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