Impact of decellularization on porcine myocardium as scaffold for tissue engineered heart tissue

Journal of Materials Science. Materials in Medicine
Xiaofeng YeQiang Zhao

Abstract

Decellularized myocardium has been proposed to construct tissue engineered heart tissue, providing the advantage of natural extracellular architecture. Various decellularization protocols have been developed, but the impact of individual decellularization reagent in the protocol remains unclear. The aim of this study is to evaluate the structural impact of three commonly used decellularization reagents on the porcine myocardium. We decellularized porcine heart tissue with trypsin, Triton X-100 or SDS, and analyzed the morphological characteristics of the remaining tissue by SEM, AFM and two-photon LSM. We further recellularized the scaffold with rat myocardial fibroblasts and cardiomyocytes separately. According to the H&E staining and DNA quantification, SDS decellularized more efficiently in comparison to the other two reagents. Moreover, we found distinct surface microarchitecture differences among groups. The changed structure of tissue might result in varied proliferation myocardial fibroblasts and biophysical performance of the engineered heart tissue. This study demonstrated that the microstructure of decellularized porcine heart tissue vary with decellularization agents. Compared to trypsin and Triton X-100, SDS not onl...Continue Reading

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Citations

Oct 9, 2018·Biotechnology Progress·Rajesh Guruswamy Damodaran, Patrick Vermette
Aug 21, 2019·Journal of Biomedical Materials Research. Part a·Jing HeQiang Ao
Feb 27, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mariana I NevesCristina C Barrias
Jul 22, 2020·Advanced Healthcare Materials·Alperen Abaci, Murat Guvendiren

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