Impact of Dental Pulp Stem Cells Overexpressing Hepatocyte Growth Factor after Cerebral Ischemia/Reperfusion in Rats

Molecular Therapy. Methods & Clinical Development
Kota SowaTakashi Okada

Abstract

Hepatocyte growth factor (HGF) has neuroprotective effects against ischemia-induced injuries. Dental pulp stem cell (DPSC) transplantation attenuates tissue injury in the brain of rats with post-transient middle cerebral artery occlusion. We sought to determine whether DPSCs that overexpress HGF can enhance their therapeutic effects on brain damage post-ischemia/reperfusion injury. Treatment with DPSCs overexpressing HGF reduced infarct volumes compared to unmodified DPSC treatment at 3 and 7 days post-transient middle cerebral artery occlusion. The use of unmodified DPSCs and DPSCs overexpressing HGF was associated with improved motor function compared to that with administration of vehicle at 7 days post-transient middle cerebral artery occlusion. DPSCs overexpressing HGF significantly inhibited microglial activation and pro-inflammatory cytokine production along with suppression of neuronal degeneration. Post-reperfusion, DPSCs overexpressing HGF attenuated the decreases in tight junction proteins, maintained blood-brain barrier integrity, and increased microvessel density in peri-infarct areas. The administration of DPSCs overexpressing HGF during the acute phase of stroke increased their neuroprotective effects by modulati...Continue Reading

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Citations

May 22, 2019·Frontiers in Neurology·Maria R GanchevaSimon A Koblar
Jan 10, 2020·Current Drug Targets·Samira AsgharzadeFatemeh Forouzanfar
Sep 18, 2020·International Journal of Molecular Sciences·Satoshi SudaKazumi Kimura
Apr 2, 2020·Current Stem Cell Research & Therapy·Xiaojie BaiHong Zhang
May 4, 2021·Frontiers in Cellular Neuroscience·Xi-Le ZhangGuo-Qing Zheng
May 11, 2021·Frontiers in Cellular Neuroscience·Hua ZhuXiaoxing Xiong

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Methods Mentioned

BETA
ELISAs
ELISA
enzyme-linked immunosorbent assays
Enzyme-Linked Immunosorbent
transfections
PCR

Software Mentioned

ImageJ
JMP9

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