Impact of devascularization and tissue procurement on cell number and RNA integrity in prostatectomy tissue

The Prostate
Rochelle Payne OndracekJames R Marshall

Abstract

Minimizing the time between tissue devascularization in robot-assisted laparoscopic radical prostatectomy (RALP) and tissue procurement should produce the highest quality tissue for research study. This study examines the relationship between intra-operative time and two indicators of tissue integrity: number of epithelial cells per gram of tissue and RNA integrity numbers (RINs). The study also compares the RIN values of tissue obtained intra-operatively by biopsy, before and after devascularization, to those from RALP specimen tissue, obtained through the routine research tissue procurement process. Prostate tissues from two series of patients were analyzed. In the first, tissue from 18 patients undergoing RALP was analyzed for number of epithelial cells per gram of tissue. In the second, RIN values of tissue from 46 patients involved in a clinical study were analyzed. RIN values were assessed from RALP specimen tissue as well as tissue removed intra-operatively by biopsy, before and after devascularization. Time from RALP to tissue procurement was not significantly associated with number of epithelial cells per gram of tissue or with RIN values. RINs of biopsy tissue obtained intra-operatively before and after devascularizat...Continue Reading

References

Nov 5, 2002·The American Journal of Pathology·Atreya DashMark A Rubin
Jul 11, 2006·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Daniel W LinPeter S Nelson
Feb 11, 2009·The Prostate·Carl MorrisonJames L Mohler
Jul 10, 2010·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Carmela RicciardelliUNKNOWN Australian Prostate Cancer BioResource

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Citations

May 28, 2019·Journal of Robotic Surgery·Anssi PetasAntti Rannikko

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