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Impact of DNA extraction, sample dilution, and reagent contamination on 16S rRNA gene sequencing of human feces

Applied Microbiology and Biotechnology

Jan 1, 2018

Eliana P. Velasquez-MejiaJuan S Escobar

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Abstract

Culture-independent methods have granted the possibility to study microbial diversity in great detail, but technical issues pose a threat to the accuracy of new findings. Biases introduced during DNA extraction can result in erroneous representations of the microbial community, particul...read more

Mentioned in this Paper

RNA, Ribosomal, 16S
Evaluation
Microbial Consortia
Extraction
Genome Sequencing
Chemical Extraction
DNA, Bacterial
Sequence Determinations, DNA
DNA Contamination
Bacterial 16S RNA
14
48
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Impact of DNA extraction, sample dilution, and reagent contamination on 16S rRNA gene sequencing of human feces

Applied Microbiology and Biotechnology

Jan 1, 2018

Eliana P. Velasquez-MejiaJuan S Escobar

PMID: 29079861

DOI: 10.1007/s00253-017-8583-z

Abstract

Culture-independent methods have granted the possibility to study microbial diversity in great detail, but technical issues pose a threat to the accuracy of new findings. Biases introduced during DNA extraction can result in erroneous representations of the microbial community, particul...read more

Mentioned in this Paper

RNA, Ribosomal, 16S
Evaluation
Microbial Consortia
Extraction
Genome Sequencing
14
48

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