Impact of drying processes on Bryophyllum pinnatum phenolic constituents and its anti-inflammatory and antioxidative activities in human erythrocytes.

Journal of Food Biochemistry
Olasunkanmi S OmojokunAline A Boligon

Abstract

The effect of drying on the phytoconstituents, antioxidative, and anti-inflammatory properties of Bryophyllum pinnatum leaves was investigated. The phenolic constituents were characterized using HPLC-DAD. The aqueous extraction was done and various assays (Inhibition of membrane stabilization, albumin Denaturation and heat-induced hemolysis, malondialdehyde (MDA), and reduced glutathione (GSH) contents, as well as superoxide dismutase (SOD) activity), were carried out on human erythrocytes. The fresh portion (89.12 µg/ml) exhibited the highest potential to inhibit heat-induced hemolysis compared to the standard drug-Diclofenac (91.51 µg/ml). Freeze-dried sample showed the highest inhibitory potential on albumin denaturation ([Freeze-dried-330.72 µg/ml], [Diclofenac-318.63 µg/ml]) and membrane destabilization ([Freeze-dried-331.93 µg/ml], [Diclofenac-289.57 µg/ml]) when compared with Diclofenac. Similarly, the freeze-dried sample showed the highest GSH and SOD level and lowest MDA level when human erythrocytes challenged with tertiary butyl hydroperoxide (tBHP) were treated with the extract. This study confirms the retention of a considerable quantity of bioactive constituents of plants when freeze-dried. PRACTICAL APPLICATIONS:...Continue Reading

References

Jun 1, 1979·Analytical Biochemistry·H OhkawaK Yagi
Jan 1, 1995·Annual Review of Biochemistry·I Fridovich
Nov 1, 1995·Australian Veterinary Journal·G P Reppas
Mar 23, 2000·Fitoterapia·D A Akinpelu
Oct 18, 2000·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·H W ChenC K Lii
May 1, 1959·Archives of Biochemistry and Biophysics·G L ELLMAN
Aug 16, 2005·Archives of Medical Research·Cyril Petibois, Gérard Déléris
Feb 5, 2008·Clinica Chimica Acta; International Journal of Clinical Chemistry·M Y Burak Cimen
Aug 16, 2008·Hematology·Michele Ana Flores ChavesAguinaldo José do Nascimento
Mar 11, 2011·Pathophysiology : the Official Journal of the International Society for Pathophysiology·Semantee BhattacharyaParames C Sil
Mar 22, 2012·BMC Research Notes·Simplice Joel Ndendoung TatsimoPierre Tane
May 26, 2012·Pathophysiology : the Official Journal of the International Society for Pathophysiology·Anandita Roy, Parames C Sil
Sep 15, 2017·Journal of Pediatric Neurosciences·Namit SinghalRajiv Tangri

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Aug 28, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ifeanyi D NwachukwuRotimi E Aluko

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