Impact of early colonizers on in vitro subgingival biofilm formation

PloS One
Thomas W AmmannT Thurnheer

Abstract

The aim of this study was to investigate the impact of early colonizing species on the structure and the composition of the bacterial community developing in a subgingival 10-species biofilm model system. The model included Streptococcus oralis, Streptococcus anginosus, Actinomycesoris, Fusobacterium nucleatum subsp. nucleatum, Veillonella dispar, Campylobacter rectus, Prevotella intermedia, Porphyromonas gingivalis, Tannerella forsythia, and Treponema denticola. Based on literature, we considered Streptococcus oralis, Streptococcus anginosus, and Actinomyces oris as early colonizers and examined their role in the biofilms by either a delayed addition to the consortium, or by not inoculating at all the biofilms with these species. We quantitatively evaluated the resulting biofilms by real-time quantitative PCR and further compared the structures using confocal laser scanning microscopy following fluorescence in situ hybridisation. The absence of the early colonizers did not hinder biofilm formation. The biofilms reached the same total counts and developed to normal thickness. However, quantitative shifts in the abundances of individual species were observed. In the absence of streptococci, the overall biofilm structure appeared...Continue Reading

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Citations

Apr 29, 2014·BMC Genomics·Alfonso Benítez-PáezAlex Mira
Jun 21, 2014·Archives of Oral Biology·Thomas ThurnheerNagihan Bostanci
Oct 2, 2014·BMC Microbiology·Kai BaoNagihan Bostanci
Jan 23, 2016·Biofouling·Yuri Wanderley CavalcantiDavid W Williams
Aug 26, 2015·Virulence·G N BelibasakisNagihan Bostanci
Mar 24, 2016·Trends in Microbiology·Henriette L RøderMette Burmølle
Apr 2, 2015·Virulence·Georgios N Belibasakis, Eleftherios Mylonakis
Dec 9, 2014·Virulence·Thomas Thurnheer, Georgios N Belibasakis
May 1, 2015·Anaerobe·Maribasappa KarchedSirkka Asikainen
Apr 7, 2017·Molecular Oral Microbiology·Susanne BlochChristina Schäffer
Dec 13, 2016·Photodiagnosis and Photodynamic Therapy·Lívia Jacovassi TavaresErica Dorigatti de Avila
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Methods Mentioned

BETA
PCR

Software Mentioned

MOWIOL
SPSS Statistics
Imaris
NoStrNoAori
NoStr

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