Impact of engagement of FcepsilonRI and CC chemokine receptor 1 on mast cell activation and motility

The Journal of Biological Chemistry
Masako TodaSanta Jeremy Ono

Abstract

CC chemokines participate in the recruitment and activation of immune cells through CC chemokine receptors (CCRs). Here, we report that cross-talk between CCR1-mediated signaling pathway and FcepsilonRI-mediated signaling pathway affects degranulation positively but affects chemotaxis of mast cells adversely. Costimulation via FcepsilonRI engagement with IgE/antigen and CCR1 engagement with recombinant human CCL3 synergistically enhanced degranulation in rat basophilic leukemia-2H3 cells expressing human CCR1 (RBL-CCR1). Interestingly, FcepsilonRI engagement inhibited CCL3-mediated chemotaxis and membrane ruffling of RBL-CCR1 cells. Small GTP-binding proteins of the Rho family, Rac, Cdc42, and Rho control chemotaxis by mediating the reorganization of the actin cytoskeleton. Both a Rho inhibitor C3 exoenzyme and a Rho kinase (ROCK) inhibitor Y-27632 inhibited chemotaxis of RBL-CCR1 cells toward CCL3, indicating that activation of the Rho/ROCK signaling pathway is required for the CCL3-mediated chemotaxis of the cells. Costimulation with IgE/antigen and CCL3 enhanced Rac and Cdc42 activation but decreased ROCK activation in RBL-CCR1 cells compared with that in the cells stimulated with CCL3 alone. These results suggest that costi...Continue Reading

References

Sep 1, 1992·The Journal of Experimental Medicine·R AlamJ A Grant
Feb 7, 1998·Science·A Hall
Jun 12, 1998·The Journal of Cell Biology·W E AllenG E Jones
Oct 28, 1998·The Journal of Clinical Investigation·H W WangP H McNeil
Oct 27, 1999·Journal of Clinical Immunology·M E RothenbergP S Foster
Mar 29, 2000·The Journal of Biological Chemistry·R M RichardsonR Snyderman
Apr 25, 2001·Clinical Immunology : the Official Journal of the Clinical Immunology Society·C Tkaczyk, A M Gilfillan
Aug 8, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·T IshizukaM Mori
Oct 23, 2001·Methods in Enzymology·Valerie Benard, Gary M Bokoch
Mar 22, 2002·Nature Reviews. Immunology·N W Lukacs
Mar 26, 2002·Immunity·Muriel LaffargueMatthias P Wymann
May 22, 2002·The Journal of Cell Biology·Takahiro TsujiShuh Narumiya
Sep 10, 2002·Methods : a Companion to Methods in Enzymology·Mario MelladoJosé Miguel Rodríguez-Frade
Oct 3, 2002·Nature Reviews. Immunology·Toshiaki Kawakami, Stephen J Galli
Nov 5, 2002·Biochemical and Biophysical Research Communications·Chang-Hoon WooJae-Hong Kim
Jan 29, 2003·The Journal of Cell Biology·Supriya SrinivasanHenry R Bourne
Feb 20, 2003·The Journal of Allergy and Clinical Immunology·Calman Prussin, Dean D Metcalfe
Apr 19, 2003·The Journal of Biological Chemistry·Renee L DoughmanRichard A Anderson
Jun 6, 2003·The Journal of Allergy and Clinical Immunology·Santa Jeremy OnoMasako Toda
Dec 6, 2003·Science·Anne J RidleyAlan Rick Horwitz

❮ Previous
Next ❯

Citations

Oct 2, 2008·Immunologic Research·Alasdair M GilfillanDean D Metcalfe
Aug 31, 2012·Journal of Medicinal Chemistry·James Pease, Richard Horuk
Oct 21, 2010·Human Reproduction Update·F M MenziesS M Nelson
Jul 14, 2009·International Immunology·Nimita H FifadaraSanta Jeremy Ono
Sep 25, 2012·Experimental and Molecular Pathology·Chuan-Hui KuoSanta Jeremy Ono
May 15, 2007·Immunological Reviews·Ulrich BlankYutaka Kanamaru
Jan 29, 2011·The Biochemical Journal·James E Pease
Jan 4, 2013·Current Eye Research·Daniel R SabanJerry Y Niederkorn
Jul 19, 2005·Expert Opinion on Investigational Drugs·James E Pease, Richard Horuk
May 15, 2013·The American Journal of Pathology·Dai MiyazakiYoshitsugu Inoue
Dec 14, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Chuan-Hui KuoSanta J Ono
May 16, 2009·Expert Opinion on Therapeutic Patents·James E Pease, Richard Horuk
Oct 8, 2011·Immunology·Laura D BennettNathalie Signoret
Aug 14, 2012·Current Opinion in Allergy and Clinical Immunology·Murat T Irkec, Banu Bozkurt
Nov 6, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Maggie M K LeeYung H Wong
Jul 13, 2006·The Journal of Biological Chemistry·Ido BacheletFrancesca Levi-Schaffer
Sep 17, 2016·International Journal of Oncology·Shujie ChengDaniel Sliva
Oct 18, 2014·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·David I KasaharaStephanie A Shore
Jul 7, 2019·The Journal of Allergy and Clinical Immunology·Ofir KleinRonit Sagi-Eisenberg
Mar 26, 2021·Current Opinion in Immunology·Pia Lazki-HagenbachRonit Sagi-Eisenberg
Aug 26, 2006·Immunology and Allergy Clinics of North America·Christine TkaczykAlasdair M Gilfillan
Oct 9, 2007·Immunology Letters·Hye Sun Kuehn, Alasdair M Gilfillan

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.