PMID: 11319163Apr 25, 2001Paper

Impact of epididymal maturation on the tyrosine phosphorylation patterns exhibited by rat spermatozoa

Biology of Reproduction
Beverley Lewis, R J Aitken

Abstract

As mammalian spermatozoa migrate through the epididymis, they acquire functionality characterized by the potential to express coordinated movement and the competence to undergo capacitation. The mechanisms by which spermatozoa gain the ability to capacitate during epididymal transit are poorly understood. The purpose of this study was to investigate the impact of epididymal maturation on the signal transduction pathways regulating tyrosine phosphorylation, because this process is thought to be central to the attainment of a capacitated state and expression of hyperactivated motility. Western blot and immunocytochemical analyses demonstrated that epididymal maturation in vivo is associated with a progressive loss of phosphotyrosine residues from the sperm head. As cells pass from the caput to the cauda epididymis, tyrosine phosphorylation becomes confined to a narrow band at the posterior margin of the acrosomal vesicle. Epididymal maturation of rat spermatozoa was also associated with an acquired competence to respond to high levels of intracellular cAMP by phosphorylating tyrosine residues on the sperm tail. Immature caput spermatozoa were incapable of exhibiting this response, despite the apparent availability of cAMP and pro...Continue Reading

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Citations

Sep 19, 2002·Molecular Reproduction and Development·Gizela GeussovaJana Peknicova
Aug 2, 2008·Reproduction, Fertility, and Development·Carmen ColasTeresa Muiño-Blanco
May 21, 2004·Biology of Reproduction·Judith SeligmanNechama S Kosower
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Mar 13, 2003·Redox Report : Communications in Free Radical Research·Jennifer D Ly, Alfons Lawen
Nov 28, 2006·Biochemical and Biophysical Research Communications·Visesato MorTapati Chatterjee
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Jan 27, 2004·Journal of Experimental Zoology. Part A, Comparative Experimental Biology·Liga BennettsR John Aitken
Feb 18, 2003·Molecular Reproduction and Development·Mark A BakerR John Aitken
May 10, 2017·Omics : a Journal of Integrative Biology·Rahim Dad Brohi, Li-Jun Huo
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Oct 25, 2017·Cell and Tissue Research·Jana JankovičováJana Antalíková
Mar 26, 2021·Computational Biology and Chemistry·Xue LiYahui Li

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