Impact of Epithelial-Stromal Interactions on Peritumoral Fibroblasts in Ductal Carcinoma in Situ

Journal of the National Cancer Institute
Carina StrellArne Östman

Abstract

A better definition of biomarkers and biological processes related to local recurrence and disease progression is highly warranted for ductal breast carcinoma in situ (DCIS). Stromal-epithelial interactions are likely of major importance for the biological, clinical, and pathological distinctions between high- and low-risk DCIS cases. Stromal platelet derived growth factor receptor (PDGFR) was immunohistochemically assessed in two DCIS patient cohorts (n = 458 and n = 80). Cox proportional hazards models were used to calculate the hazard ratios of recurrence. The molecular mechanisms regulating stromal PDGFR expression were investigated in experimental in vitro co-culture systems of DCIS cells and fibroblasts and analyzed using immunoblot and quantitative real-time PCR. Knock-out of JAG1 in DCIS cells and NOTCH2 in fibroblasts was obtained through CRISPR/Cas9. Experimental data were validated by mammary fat pad injection of DCIS and DCIS-JAG1 knock-out cells (10 mice per group). All statistical tests were two-sided. PDGFRα(low)/PDGFRβ(high) fibroblasts were associated with increased risk for recurrence in DCIS (univariate hazard ratio = 1.59, 95% confidence interval [CI] = 1.02 to 2.46; P = .04 Wald test; multivariable hazard r...Continue Reading

References

Apr 3, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Anne Vincent-SalomonUNKNOWN breast cancer study group of the Institut Curie
May 6, 2008·Cancer Cell·Min HuKornelia Polyak
Feb 4, 2009·Breast Cancer Research : BCR·Xiao-Jun MaDennis C Sgroi
Feb 13, 2009·Current Opinion in Genetics & Development·Arne Ostman, Martin Augsten
Feb 17, 2009·Proceedings of the National Academy of Sciences of the United States of America·Min HuKornelia Polyak
May 29, 2009·BMC Bioinformatics·Weijun LuoPeter J Woolf
Jun 9, 2009·Cancer Biology & Therapy·Agnieszka K WitkiewiczMichael P Lisanti
Jan 15, 2010·Journal of the National Cancer Institute·Beth A VirnigRobert L Kane
Feb 18, 2011·Disease Models & Mechanisms·Thomas R Cox, Janine T Erler
May 4, 2011·Breast Cancer Research and Treatment·Ragnhild Sørum FalkTor Haldorsen
Nov 5, 2011·Breast Cancer Research and Treatment·Clint E JohnsonIan G Campbell
Nov 6, 2012·Biochimica Et Biophysica Acta·Timothy MarshSandra S McAllister
Jan 25, 2013·Cancer Research·Thomas R CoxJanine T Erler
Jul 31, 2013·Molecular Oncology·Catherine F CowellJorge S Reis-Filho
Nov 28, 2013·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·E KarlssonF Wärnberg
Feb 4, 2014·International Journal of Breast Cancer·Wenjing ZhouFredrik Wärnberg
May 2, 2014·Nature Reviews. Drug Discovery·Emma R Andersson, Urban Lendahl
Aug 2, 2014·Nature Cell Biology·Sandra S McAllister, Robert A Weinberg
Nov 13, 2014·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Rebecca A PrevisAnil K Sood
Aug 25, 2015·Nature Cell Biology·Maria-Giuseppina ProcopioG Paolo Dotto
Apr 14, 2016·Stem Cell Reports·Eike-Benjamin BrauneUrban Lendahl
Aug 24, 2016·Nature Reviews. Cancer·Raghu Kalluri
Nov 20, 2016·Journal of Clinical Pathology·Joe YeongJabed Iqbal
Feb 1, 2017·The Journal of Pathology. Clinical Research·Janna PaulssonArne Östman
Feb 10, 2017·International Journal of Epidemiology·Marike GabrielsonPer Hall
Feb 25, 2017·The Journal of Experimental Medicine·Daniel ÖhlundDavid A Tuveson
Jun 28, 2017·Cancer Discovery·Carlos R Gil Del AlcazarKornelia Polyak
Sep 25, 2017·Journal of Internal Medicine·C-H HeldinB Westermark

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Citations

Apr 7, 2020·Expert Opinion on Therapeutic Targets·Rosamaria LappanoErnestina Marianna De Francesco
Jan 26, 2020·Nature Reviews. Cancer·Erik SahaiZena Werb
Jul 16, 2020·Breast Cancer Research : BCR·Claire BonneauFatima Mechta-Grigoriou
Aug 13, 2020·International Journal of Molecular Sciences·Cristiana TanaseMihail Eugen Hinescu
Dec 10, 2020·Nature Reviews. Drug Discovery·Samarpan MajumderLucio Miele
May 22, 2020·Cancer Discovery·Yann KiefferFatima Mechta-Grigoriou
Jul 3, 2019·Biochimica Et Biophysica Acta. Reviews on Cancer·Maximilian BoeschMartin H Brutsche
Jan 17, 2021·Proceedings of the National Academy of Sciences of the United States of America·Mehdi DamaghiRobert J Gillies
Jan 28, 2021·Cancers·Géraldine Gentric, Fatima Mechta-Grigoriou
Jan 6, 2021·The Journal of Immunology : Official Journal of the American Association of Immunologists·Alfie T BakerAnne L Fletcher
Apr 4, 2021·International Journal of Molecular Sciences·Dina V AntonovaVictor V Pleshkan
Apr 17, 2021·Seminars in Cancer Biology·Antonio Ferreira, Jon C Aster
May 22, 2021·Journal of Experimental & Clinical Cancer Research : CR·Freja Albjerg VenningChris Denis Madsen
Jun 6, 2021·The British Journal of Surgery·Andreas KarakatsanisUNKNOWN SentiNot Trialists Group
May 7, 2021·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Carina StrellArne Östman
Jul 1, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Meng-Xi XiuBo-Hai Kuang

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