Abstract
Aim:
This work studied the impact of the quorum-sensing molecule, farnesol (FAR), on fluconazole (FLC)-resistant Candida albicans isolate CY 1123 compared with the susceptible standard strain C. albicans SC5314. The genes encoding efflux pumps belonging to the ATP-binding cassette (ABC) and major facilitator superfamilies, together with overexpression or point mutation of the ERG11 gene, are the main resistance mechanisms to azole antifungal drugs.
Results:
The upregulation of genes coding for CDR1, CDR2, and MDR1 were confirmed by qPCR with respect to the housekeeping gene ACT1 in the resistant strain. The contribution of the ERG11 gene was also observed. Markedly, increased pump activity (Cdr1 and/or Cdr2) in the CY 1123 strain was confirmed using diS-C3(3) assay. However, the addition of FAR to the yeasts diminished the difference in staining levels between the SC5314 and CY 1123 strains, demonstrating the concentration-dependent character that could be caused by an effective modulation of Cdr pumps. FAR (60 and 100 μM) was also able to decrease the minimal inhibitory concentrations (MIC50), denoting the inhibition of planktonic cells by 50%, from 8 to 4 μg/mL of FLC when the resistant strain CY 1123 was not cultivated wi...Continue Reading
References
Dec 31, 1997·AIDS·J D CartledgeB G Gazzard
Sep 24, 1998·Current Genetics·V GuptaR Prasad
Nov 4, 2000·Expert Opinion on Investigational Drugs·H L HoffmanM E Klepser
Jun 27, 2001·Applied and Environmental Microbiology·J M HornbyK W Nickerson
Apr 5, 2002·Phytotherapy Research : PTR·Mary H Grace
Oct 31, 2002·Applied and Environmental Microbiology·Gordon RamageJosé L López-Ribot
Apr 17, 2003·The Journal of Antimicrobial Chemotherapy·Maria Agnes DawisStephen G Jenkins
Apr 24, 2003·Antimicrobial Agents and Chemotherapy·Christian GauthierMartine Raymond
Jun 14, 2003·The Journal of Antimicrobial Chemotherapy·F C Odds
Jul 25, 2003·Antimicrobial Agents and Chemotherapy·Dominique SanglardJacques Bille
Sep 19, 2003·International Journal of Antimicrobial Agents·Manuela Schuetzer-MuehlbauerKarl Kuchler
Oct 18, 2006·FEMS Yeast Research·Mary Ann Jabra-RizkTimothy Meiller
Mar 12, 2008·Antimicrobial Agents and Chemotherapy·K WeberM Ruhnke
Aug 20, 2008·Antimicrobial Agents and Chemotherapy·Ann R HolmesRichard D Cannon
Apr 16, 2009·Clinical Microbiology Reviews·Richard D CannonBrian C Monk
May 1, 2009·Annals of Clinical Microbiology and Antimicrobials·Lorena S DerengowskiIldinete Silva-Pereira
Nov 23, 2011·Antimicrobial Agents and Chemotherapy·Li-Hua YuShuang-Bo Xu
Feb 9, 2012·Journal of Amino Acids·Rajendra PrasadManpreet Kaur Rawal
Jun 21, 2012·Medical Mycology·Rossana A CordeiroMarcos F G Rocha
Feb 13, 2013·BMC Infectious Diseases·Andargachew MuluEmiko Isogai
Jul 24, 2013·BioMed Research International·Claudia Spampinato, Darío Leonardi
May 14, 2014·Mikrobiyoloji bülteni·Sinem Gulat, Mine Doluca Dereli
Oct 8, 2014·The Journal of Antimicrobial Chemotherapy·Aspasia KatragkouThomas J Walsh
Dec 17, 2014·Antimicrobial Agents and Chemotherapy·Yali LiRuoyu Li
Mar 26, 2015·Frontiers in Microbiology·Joanna SzczepaniakAnna Krasowska
Apr 18, 2015·Annals of Clinical Microbiology and Antimicrobials·Mohamed El-AziziNancy Khardori
Dec 10, 2015·Mycoses·Arati ManeArun Risbud
Apr 30, 2016·Jundishapur Journal of Microbiology·Kamiar ZomorodianAli Poostforoush Fard
Mar 2, 2017·Current Genetics·Melanie Polke, Ilse D Jacobsen
Jul 13, 2017·PloS One·Yohsuke NagayoshiKoichi Izumikawa
Citations
May 12, 2020·PloS One·Vartika Srivastava, Aijaz Ahmad
Jul 28, 2020·Frontiers in Microbiology·Jiangyan YongYan Li
May 10, 2020·Scientific Reports·H M H N BandaraL P Samaranayake
Sep 11, 2019·Antimicrobial Agents and Chemotherapy·Michel MonodTsuyoshi Yamada
Oct 23, 2020·FEBS Letters·Evan R Buechel, Heather W Pinkett
Jan 30, 2021·Journal of Global Antimicrobial Resistance·Haisheng ChenWenna Shi