Impact of Fishmeal Replacement in Diets for Gilthead Sea Bream (Sparus aurata) on the Gastrointestinal Microbiota Determined by Pyrosequencing the 16S rRNA Gene

PloS One
G EstruchS Martinez-Llorens

Abstract

Recent studies have demonstrated the impact of diet on microbiota composition, but the essential need for the optimization of production rates and costs forces farms and aquaculture production to carry out continuous dietary tests. In order to understand the effect of total fishmeal replacement by vegetable-based feed in the sea bream (Sparus aurata), the microbial composition of the stomach, foregut, midgut and hindgut was analysed using high-throughput 16S rDNA sequencing, also considering parameters of growth, survival and nutrient utilisation indices.A total of 91,539 16S rRNA filtered-sequences were analysed, with an average number of 3661.56 taxonomically assigned, high-quality sequences per sample. The dominant phyla throughout the whole gastrointestinal tract were Actinobacteria, Protebacteria and Firmicutes. A lower diversity in the stomach in comparison to the other intestinal sections was observed. The microbial composition of the Recirculating Aquaculture System was totally different to that of the sea bream gastrointestinal tract. Total fishmeal replacement had an important impact on microbial profiles but not on diversity. Streptococcus (p-value: 0.043) and Photobacterium (p-value: 0.025) were highly represented i...Continue Reading

References

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Citations

Mar 11, 2016·FEMS Microbiology Ecology·Matteo SoveriniMarco Candela
Feb 9, 2017·Journal of Applied Microbiology·A M TarneckiC R Arias
May 22, 2018·Frontiers in Microbiology·Sian EgertonR Paul Ross
Nov 19, 2019·Frontiers in Microbiology·M Carla PiazzonJaume Pérez-Sánchez
Mar 13, 2021·Applied Microbiology and Biotechnology·Bernardo Veyrand-QuirósEduardo Quiroz-Guzman
Aug 7, 2021·Journal of Animal Physiology and Animal Nutrition·Arvind D DiwanArchana N Panche
Sep 2, 2021·Journal of Applied Microbiology·Ernesto Larios-SorianoMario A Galaviz
Dec 3, 2021·The Science of the Total Environment·Lu-Xi HeGuang-Guo Ying

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Methods Mentioned

BETA
PCR
DNA assay
scraping

Software Mentioned

Statistical Analysis of Metagenomic Profiles STAMP
STAMP
QIIME
UniFrac
Statgraphics Centurion
RAST
UCHIME
MG

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