Impact of genetic variation on synaptic protein levels in genetically diverse mice

Proteomics
Maarten LoosSabine Spijker

Abstract

The relative abundance of synaptic proteins shapes protein complex formation and is essential for synapse function and behavioral fitness. Here, we have used a panel of highly diverse inbred strains of mice-NOD/LtJ, A/J, 129S1/SvImJ, FVB/NJ, C57BL/6J, WSB/EiJ, PWK/PhJ, and CAST/EiJ-to quantify the effects of genetic variation on the synaptic proteome between strains. Using iTRAQ-based quantitative proteome analyses, we detected significant differences in ∼20% of 400 core synaptic proteins. Surprisingly, the differentially abundant proteins showed a modest range of variation across strains, averaging about 1.3-fold. Analysis of protein abundance covariation across the eight strains identified known protein-protein relations (proteins of Arp2/3 complex), as well as novel relations (e.g. Dlg family, Fscn1). Moreover, covariation of synaptic proteins was substantially tighter (∼fourfold more dense than chance level) than corresponding networks of synaptic transcripts (∼twofold more dense than chance). The tight stoichiometry and coherent synaptic protein covariation networks suggest more intense evolutionary selection at this level of molecular organization. In conclusion, genetic diversity in the mouse genome differentially affect...Continue Reading

References

May 2, 1997·The Journal of Biological Chemistry·M TakeuchiY Takai
Jul 29, 2003·Proceedings of the National Academy of Sciences of the United States of America·John D Storey, Robert Tibshirani
Oct 26, 2005·Nature·Iiris HovattaCarrolee Barlow
Nov 18, 2006·Cell·Shigeo TakamoriReinhard Jahn
Jan 25, 2007·Annual Review of Biochemistry·Morgan Sheng, Casper C Hoogenraad
Aug 4, 2007·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Adam RobertsDavid W Threadgill
Aug 27, 2009·PloS One·Anne-Elise TobinDavid J Schulz
Jan 12, 2010·American Journal of Human Genetics·Dina RuanoDanielle Posthuma
Jul 6, 2010·The International Journal of Biochemistry & Cell Biology·Asier Jayo, Maddy Parsons
Feb 11, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Julien AmendolaJean-Marc Goaillard
Dec 4, 2012·Nature Neuroscience·Jess NithianantharajahSeth G N Grant
Nov 19, 2014·Analytical Chemistry·Dustin C FrostLingjun Li

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Citations

Mar 14, 2017·Frontiers in Molecular Neuroscience·Sanjeev V Namjoshi, Kimberly F Raab-Graham
Jun 11, 2019·Frontiers in Molecular Neuroscience·Cory ParksMegan K Mulligan

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