Impact of heterologous expression of Escherichia coli UDP-glucose pyrophosphorylase on trehalose and glycogen synthesis in Corynebacterium glutamicum

Applied and Environmental Microbiology
Leandro PadillaEduardo Agosin

Abstract

Trehalose is a disaccharide with a wide range of applications in the food industry. We recently proposed a strategy for trehalose production based on improved strains of the gram-positive bacterium Corynebacterium glutamicum. This microorganism synthesizes trehalose through two major pathways, OtsBA and TreYZ, by using UDP-glucose and ADP-glucose, respectively, as the glucosyl donors. In this paper we describe improvement of the UDP-glucose supply through heterologous expression in C. glutamicum of the UDP-glucose pyrophosphorylase gene from Escherichia coli, either expressed alone or coexpressed with the E. coli ots genes (galU otsBA synthetic operon). The impact of such expression on trehalose accumulation and excretion, glycogen accumulation, and the growth pattern of new recombinant strains is described. Expression of the galU otsBA synthetic operon resulted in a sixfold increase in the accumulated and excreted trehalose relative to that in a wild-type strain. Surprisingly, single expression of galU also resulted in an increase in the accumulated trehalose. This increase in trehalose synthesis was abolished upon deletion of the TreYZ pathway. These results proved that UDP-glucose has an important role not only in the OtsBA ...Continue Reading

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Citations

Apr 27, 2012·The ISME Journal·Eamonn P CulliganColin Hill
Apr 26, 2011·Applied and Environmental Microbiology·Ana Lúcia CarvalhoHelena Santos
Jan 20, 2016·Applied Microbiology and Biotechnology·Gazi Sakir HossainLong Liu
Dec 1, 2015·Frontiers in Microbiology·Ana C EbrechtMiguel A Ballicora
Sep 16, 2018·Microbial Cell Factories·Jian ZhaMattheos A G Koffas
Nov 23, 2019·International Journal of Molecular Sciences·Antje KumpfDirk Tischler
Mar 7, 2021·Microorganisms·Doglas PariseJan Baumbach

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