Impact of hypoxia and hypercapnia on calcium oxalate toxicity in renal epithelial and interstitial cells.

Urological Research
Yanwei CaoThomas Knoll

Abstract

Although there is an ongoing controversy about the primary site of calcium oxalate stone (CaOx) formation, there is some evidence for extratubular crystallization. However, the mechanisms leading to such interstitial calcifications are not clear. Anatomical studies have demonstrated a close association between the renal vasculature and renal tubules. It has been hypothesized that disorders of the vasculature may contribute to renal stone formation. The exceptional papillary environment with low oxygen and high carbon dioxide is of interest in this context and its impact on CaOx toxicity to renal cells has to be evaluated. LLC-PK1, Madin-Darby canine kidney (MDCK), human umbilical vein endothelial (HUVEC) and fibroblast cell lines were exposed to hypoxia (3% O2) alone, hypercapnia combined with hypoxia (3% O2, 18% CO2) or standard culture conditions (20% O2) for 72 h. Cell survival rates were determined microscopically after 4 h of incubation with CaOx at final concentrations of 1, 2 and 4 mM. DAPI staining and western blot were used to evaluate the induction of apoptosis. We confirmed that CaOx leads to concentration-dependent effects on the viability of the cell lines. HUVECs were most vulnerable to CaOx among the four cell li...Continue Reading

References

May 1, 1978·Kidney International·B Finlayson
Dec 1, 1991·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·C Y Pak
Apr 1, 1990·The Journal of Urology·F J Sampaio, A H Aragao
May 1, 1987·The Journal of Urology·L Cifuentes DelatteJ A Medina
Dec 1, 1985·The Journal of Urology·P G WernessB Finlayson
Dec 1, 1994·Journal of the American Society of Nephrology : JASN·M IwataR A Zager
Jun 12, 1998·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·G WiegeleL B Zimmerhackl
Jun 19, 1998·Urological Research·J M Baumann
Jan 19, 2000·Toxicology and Applied Pharmacology·C MillerC Scheid
Jan 10, 2001·Current Opinion in Urology·C F Verkoelen, M S Schepers
Jun 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·H JiangJ A Powell-Coffman
Jun 4, 2002·Journal of Endourology·Hyeon Hoe KimSi Whang Kim
Aug 16, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Wensheng Zhang, Aurélie Edwards
Feb 18, 2003·International Journal of Urology : Official Journal of the Japanese Urological Association·Tatsuya NakataniTaketoshi Kishimoto
Mar 6, 2003·The Journal of Clinical Investigation·Andrew P EvanMarc Grynpas
Nov 25, 2003·Seminars in Nephrology·Pothana Saikumar, Manjeri A Venkatachalam
Apr 13, 2004·The Journal of Urology·Marshall L StollerJohn P Kane
Jun 16, 2004·Urological Research·Thomas KnollPeter Alken
Oct 4, 2005·The Journal of Clinical Investigation·Fredric L CoeElaine Worcester
Jun 1, 1937·Annals of Surgery·A Randall

❮ Previous
Next ❯

Related Concepts

Related Feeds

Brain Ischemia

Brain ischemia is a condition in which there is insufficient blood flow to the brain to meet metabolic demand. Discover the latest research on brain ischemia here.