Impact of insulin on primary arcuate neurons culture is dependent on early-postnatal nutritional status and neuronal subpopulation

PloS One
Lyvianne DecourtyeLaurent Kappeler

Abstract

Nutrition plays a critical role in programming and shaping linear growth during early postnatal life through direct action on the development of the neuroendocrine somatotropic (GH/IGF-1) axis. IGF-1 is a key factor in modulating the programming of linear growth during this period. Notably, IGF-1 preferentially stimulates axonal growth of GHRH neurons in the arcuate nucleus of the hypothalamus (Arc), which is crucial for the proliferation of somatotroph progenitors in the pituitary, thus influencing later GH secretory capacity. However, other nutrition-related hormones may also be involved. Among them, insulin shares several structural and functional similarities with IGF-1, as well as downstream signaling effectors. We investigated the role of insulin in the control of Arc axonal growth using an in vitro model of arcuate explants culture and a cell-type specific approach (GHRH-eGFP mice) under both physiological conditions (normally fed pups) and those of dietary restriction (underfed pups). Our data suggest that insulin failed to directly control axonal growth of Arc neurons or influence specific IGF-1-mediated effects on GHRH neurons. Insulin may act on neuronal welfare, which appears to be dependent on neuronal sub-populati...Continue Reading

References

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Oct 31, 2008·PLoS Biology·Laurent KappelerMartin Holzenberger
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Jan 12, 2017·PloS One·Lyvianne DecourtyeLaurent Kappeler

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Citations

Aug 1, 2020·Minerva pediatrica·Lorena MatontiFrancesco Chiarelli

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Methods Mentioned

BETA
fluorescence microscopy
ELISA

Software Mentioned

GraphPad Prism
ImageJ
NeuronJ

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