Impact of IS1111 insertion on the MLVA genotyping of Coxiella burnetii

Microbes and Infection
Karim Sidi-BoumedineElodie Rousset

Abstract

Q fever epidemiological investigations of the likely sources of contamination may involve Coxiella burnetii MLVA for direct and rapid typing from clinical samples. However, little information is available with regards to PCR amplification failures in C. burnetii MLVA typing. This paper focuses on difficulties encountered with MLVA loci that may impact the interpretation of MLVA data and shows that some loci may constitute hotspots for mutational events. MLVA genotyping, using 17 different loci, was used on vaginal swabs (VS) from clinically infected animals as described elsewhere (Chmielewski et al., 2009). Amplicons of interest were sequenced and identified using the BLAST software by comparison with sequences available in GenBank. All VS samples produced MLVA patterns. However, amplification failures or unexpected sizes amplicons (>to 1.5 kbp), making the interpretation of MLVA complicated, were also observed. Sequencing of these amplicons revealed the presence of IS1111 element insertion. In this C. burnetii MLVA study some difficulties encountered with genotyping are highlighted and the role of IS1111 element in genome plasticity is confirmed. Finally, the need for the selection of a set of VNTRs for an efficient MLVA schem...Continue Reading

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Citations

Jun 11, 2016·International Journal of Medical Microbiology : IJMM·Gemma VincentStephen Graves
May 31, 2018·BMC Veterinary Research·M Di DomenicoC Cammà
Dec 6, 2018·BMC Microbiology·Dimosthenis ChochlakisEvanthia I Petridou
Mar 30, 2017·Microbial Genomics·Sonora OlivasTalima Pearson
Mar 16, 2021·Frontiers in Cellular and Infection Microbiology·Sara TomaiuoloMarcella Mori
Jun 3, 2021·Pathogens·Claudia M HemsleyRichard W Titball

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