Impact of Leishmania donovani infection on the HLA I self peptide repertoire of human macrophages

PloS One
Lydon Wainaina NyamburaPeter Walden

Abstract

Macrophages are specialized antigen-presenting cells that process and present self-antigens for induction of tolerance, and foreign antigens to initiate T cell-mediated immunity. Despite this, Leishmania donovani (LD) are able to parasitize the macrophages and persist. The impact of this parasitizing and persistence on antigen processing and presentation by macrophages remains poorly defined. To gain insight into this, we analyzed by liquid chromatography tandem mass spectrometry (LC-MS/MS) and compared the HLA-I self-peptidomes, proteasome compositions, HLA expression and activation states of non-infected and LD-infected THP1-derived macrophages. We found that, though both HLA-I peptidomes were dominated by nonapeptides, they were heterogeneous and individualized, with differences in HLA binding affinities and anchor residues. Non-infected and LD-infected THP1-derived macrophages were able to sample peptides from source proteins of almost all subcellular locations and involved in various cellular functions, but in different proportions. In the infected macrophages, there was increased sampling of plasma membrane and extracellular proteins, and those involved in immune responses, cell communication/signal transduction and metab...Continue Reading

References

Oct 25, 1990·Nucleic Acids Research·T D Schneider, R M Stephens
Jun 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·N E ReinerW R McMaster
Aug 1, 1980·International Journal of Cancer. Journal International Du Cancer·S TsuchiyaK Tada
Aug 6, 1996·Proceedings of the National Academy of Sciences of the United States of America·G NiedermannK Eichmann
May 11, 1999·Parasitology Today·C Bogdan, M Röllinghoff
Oct 8, 1999·Lancet·B L Herwaldt
Dec 22, 1999·Immunogenetics·H RammenseeS Stevanović
Aug 9, 2001·Transactions of the Royal Society of Tropical Medicine and Hygiene·P Desjeux
Dec 18, 2001·European Journal of Immunology·G ForgetM Olivier
Apr 30, 2003·Protein Science : a Publication of the Protein Society·Morten NielsenOle Lund
Jan 22, 2004·Current Opinion in Immunology·Peter Michael Kloetzel, Ferry Ossendorp
Apr 20, 2004·Rapid Communications in Mass Spectrometry : RCM·Rodion Demine, Peter Walden
Jun 3, 2004·Genome Research·Gavin E CrooksSteven E Brenner
Apr 16, 2005·Clinical Microbiology Reviews·Martin OlivierGeneviève Forget
Dec 31, 2005·Nucleic Acids Research·Gopa R MishraAkhilesh Pandey
Jul 20, 2006·Cellular Microbiology·Robert LodgeAlbert Descoteaux
Oct 24, 2007·Trends in Biochemical Sciences·Lu Deng, Roy A Mariuzza
Sep 16, 2008·Experimental Parasitology·Liliam O FariaCezar Martins de Sá
Nov 20, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Helen M MulemeJude E Uzonna
Dec 27, 2011·Nature Immunology·Eleanor Z KincaidKenneth L Rock
Aug 8, 2012·The Journal of Biological Chemistry·Saulius JarmalaviciusPeter Walden
Jun 21, 2013·Nature Communications·Eleni AdamopoulouChristina Stoeckle
Feb 26, 2014·Trends in Immunology·Kenneth L RockAlfred L Goldberg
Jun 27, 2014·PLoS Neglected Tropical Diseases·Ermias DiroJohan van Griensven
Aug 22, 2014·PLoS Neglected Tropical Diseases·Begoña Monge-MailloRogelio López-Vélez
Sep 19, 2014·PLoS Neglected Tropical Diseases·José Angelo LindosoAna Rabello
Oct 29, 2014·Nucleic Acids Research·UNKNOWN UniProt Consortium
Dec 3, 2014·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Sarman Singh
Jul 3, 2015·Frontiers in Immunology·Christine Riedhammer, Robert Weissert
Aug 21, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Lydon Wainaina NyamburaPeter Walden
Apr 11, 2018·Molecular Immunology·Julie Boucau, Sylvie Le Gall

❮ Previous
Next ❯

Citations

Oct 23, 2019·Vaccines·Shubhranshu ZutshiBhaskar Saha
Mar 24, 2021·Parasite Immunology·Supratim PradhanBudhaditya Mukherjee

❮ Previous
Next ❯

Methods Mentioned

BETA
flow cytometry
electrophoresis

Software Mentioned

Biotools
Data Analysis
GelAnalyser
WinMDi
CellQuest
GelAnayzer2010
MASCOT
Sequit

Related Concepts

Related Feeds

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.

Related Papers

The Journal of Immunology : Official Journal of the American Association of Immunologists
Lydon Wainaina NyamburaPeter Walden
Comptes rendus de l'Académie des sciences. Série III, Sciences de la vie
P KourilskyC Jaulin
© 2022 Meta ULC. All rights reserved