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Impact of library preparation on downstream analysis and interpretation of RNA-Seq data: comparison between Illumina PolyA and NuGEN Ovation protocol

PloS One

Aug 27, 2013

Zhifu SunE Aubrey Thompson

Abstract

The sequencing by the PolyA selection is the most common approach for library preparation. With limited amount or degraded RNA, alternative protocols such as the NuGEN have been developed. However, it is not yet clear how the different library preparations affect the downstream analyses...read more

Mentioned in this Paper

Real-Time Polymerase Chain Reaction
TWIST1 protein, human
Exons
Genome
Adenine Polynucleotides
Estrogen receptor alpha, human
RNA, Untranslated
DEGS1 wt Allele
Mammary Gland
Sequence Determinations, RNA
16
73
Paper Details
References
  • References27
  • Citations7
123
  • References27
  • Citations7
1

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Impact of library preparation on downstream analysis and interpretation of RNA-Seq data: comparison between Illumina PolyA and NuGEN Ovation protocol

PloS One

Aug 27, 2013

Zhifu SunE Aubrey Thompson

PMID: 23977132

DOI: 10.1371/journal.pone.0071745

Abstract

The sequencing by the PolyA selection is the most common approach for library preparation. With limited amount or degraded RNA, alternative protocols such as the NuGEN have been developed. However, it is not yet clear how the different library preparations affect the downstream analyses...read more

Mentioned in this Paper

Real-Time Polymerase Chain Reaction
TWIST1 protein, human
Exons
Genome
Adenine Polynucleotides
Estrogen receptor alpha, human
RNA, Untranslated
DEGS1 wt Allele
Mammary Gland
Sequence Determinations, RNA
16
73

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RNA Splicing (Keystone)

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Paper Details
References
  • References27
  • Citations7
123
  • References27
  • Citations7
1

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