Impact of Mitomycin-C application time on the scleral Mitomycin-C concentration

Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics
C VassP Nimmerrichter

Abstract

The aim of this study was to determine the effect of varying the application time of Mitomycin-C (MMC) on the scleral concentration of MMC. The sclerae of 14 human donor eyes were used for this study. The episcleral sides of the 4 scleral quadrants of each donor eye were exposed for 0.5, 1, 3 and 5 min to round, 8 mm-diameter sponges soaked with 50 microl of 0.2 mg/ml MMC. After 40-ml irrigation with saline, a central 8-mm diameter scleral disk was punched out, homogenized and analyzed with high performance liquid chromatography (HPLC). The scleral MMC concentrations (microg/g) after 0.5, 1, 3 and 5 min application times were 6.40 (+/-3.38), 9.02 (+/-2.40), 12.31 (+/-3.37), and 13.97 (+/-3.83). The differences of scleral MMC concentration in paired t-tests were statistically significant comparing 0.5 with 1 and 1 with 5 min application. However the effect was relatively small within the range of usual application times (1 to 5 min), and 64% of the MMC was delivered to the sclera within the first min.

References

Dec 1, 1991·Archives of Ophthalmology·Y KitazawaM Minobe
Mar 1, 1991·Ophthalmology·S S Palmer
Jan 1, 1990·Journal of Ocular Pharmacology·C W ChenC C Lee
May 15, 1994·American Journal of Ophthalmology·T Yamamoto, Y Kitazawa
Sep 15, 1993·American Journal of Ophthalmology·P T ZachariaJ S Schuman
Aug 1, 1997·Archives of Ophthalmology·A L RobinR Bhatnagar
Apr 1, 1995·Journal of Glaucoma·W J FlynnS L Bifano

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