Abstract
A microbial community was cultivated in flow cells with 2,4,6-trichlorobenzoic acid (2,4,6-TCB) as sole carbon and energy source and was examined with scanning confocal laser microscopy and fluorescent molecular probes. The biofilm community which developed under these conditions exhibited a characteristic architecture, including a basal cell layer and conspicuous mounds of bacterial cells and polymer (approximately 20 to 30 (mu)m high and 25 to 40 (mu)m in diameter) occurring at 20- to 200-(mu)m intervals. When biofilms grown on 2,4,6-TCB were shifted to a labile, nonchlorinated carbon source (Trypticase soy broth), the biofilms underwent an architectural change which included the loss of mound structures and the formation of a more homogeneous biofilm. Neutrally charged fluorescent dextrans, which upon hydration become cationic, were observed to bind to mounds, as well as to the basal cell layer, in 14-day biofilms. In contrast, polyanionic dextrans bound only to the basal cell layer, indicating that this material incorporated sites with both positive and negative charge. The results from this study indicate that nutrient composition has a significant impact on both the architecture and the physicochemistry of degradative bio...Continue Reading
References
Jul 1, 1991·Applied and Environmental Microbiology·J T GrotenhuisA J Zehnder
Oct 1, 1991·Journal of Bacteriology·J R LawrenceD E Caldwell
Jan 1, 1987·Annual Review of Microbiology·J W CostertonT J Marrie
Jan 1, 1986·Annual Review of Microbiology·S R HutchinsC L Brierley
Jan 1, 1985·Annual Review of Microbiology·I W Sutherland
Jan 1, 1983·Critical Reviews in Microbiology·I W Sutherland
Feb 1, 1995·Applied and Environmental Microbiology·A A Massol-DeyáJ M Tiedje
Feb 1, 1995·Journal of Bacteriology·G A JamesJ W Costerton
Nov 1, 1994·Journal of Bacteriology·H M DaltonK C Marshall
Apr 1, 1994·Journal of Bacteriology·J W CostertonG James
May 1, 1993·Applied and Environmental Microbiology·M WagnerK H Schleifer
Jan 1, 1995·Annual Review of Microbiology·J W CostertonH M Lappin-Scott
May 1, 1996·Applied and Environmental Microbiology·H J HarmsenW M de Vos
Dec 1, 1996·Applied and Environmental Microbiology·S MøllerS Molin
Jan 1, 1983·Applied and Environmental Microbiology·D J Uhlinger, D C White
Sep 1, 1985·Applied and Environmental Microbiology·R ConradJ G Zeikus
Feb 1, 1994·Applied and Environmental Microbiology·G M WolfaardtD E Caldwell
May 1, 1994·Applied and Environmental Microbiology·D R KorberJ W Costerton
May 1, 1994·Microbial Ecology·G M WolfaardtD E Caldwell
Citations
Oct 6, 2000·Microbiology·A HeydornS Molin
Nov 30, 2005·Biotechnology and Bioengineering·John KomlosRobert R Sharp
Feb 4, 2009·Journal of Applied Microbiology·W FangS L Ong
Dec 1, 1998·Journal of Applied Microbiology·C M BuswellS A Leach
Dec 1, 1998·Journal of Applied Microbiology·P StoodleyH M Lappin-Scott
May 10, 2002·Environmental Microbiology·James E M Stach, Richard G Burns
Apr 27, 2002·Applied and Environmental Microbiology·Bjarke B ChristensenSøren Molin
Feb 3, 2005·Microbial Ecology·J KomlosR R Sharp
Oct 6, 2000·Microbiology·A HeydornS Molin
Apr 16, 2003·Journal of Applied Microbiology·O TresseD Rho
Mar 26, 2010·Biofouling·C S SrinandanA S Nerurkar
Apr 22, 2010·Environmental Microbiology·Johanna S AndrewsSteven A Banwart
Aug 14, 2001·Infection and Immunity·R J PalmerP E Kolenbrander
Dec 8, 2005·FEMS Microbiology Ecology·Thomas J AsprayRichard G Burns
Sep 8, 2006·Applied and Environmental Microbiology·Kristin E SearcyThomas Harter
Jun 13, 2009·FEMS Microbiology Ecology·Anne BöhmeKirsten Küsel
Apr 1, 2008·FEMS Microbiology Ecology·Philip BreugelmansDirk Springael
Jun 19, 2015·PloS One·Chase KernanJean Huang
Oct 10, 2009·Journal of Medical Microbiology·Christian HannigAli Al-Ahmad
Feb 27, 2015·Clinical Oral Investigations·Aldo del Carpio-PerochenaMarco Hungaro Duarte
Jan 5, 2008·Biotechnology and Bioengineering·G M WolfaardtM Pilaski
Oct 1, 2018·Acta Biomaterialia·Bo HuangYoav Finer
Feb 17, 1998·Applied and Environmental Microbiology·S MøllerS Molin
Jul 23, 2019·Biomaterials Science·Haw-Young KwonYoung-Tae Chang
Sep 8, 2010·Journal of Microbiological Methods·Tom Coenye, Hans J Nelis
Sep 22, 2010·Journal of Microbiological Methods·K WoutersD Springael