Impact of nutrient composition on a degradative biofilm community.

Applied and Environmental Microbiology
S MollerD E Caldwell

Abstract

A microbial community was cultivated in flow cells with 2,4,6-trichlorobenzoic acid (2,4,6-TCB) as sole carbon and energy source and was examined with scanning confocal laser microscopy and fluorescent molecular probes. The biofilm community which developed under these conditions exhibited a characteristic architecture, including a basal cell layer and conspicuous mounds of bacterial cells and polymer (approximately 20 to 30 (mu)m high and 25 to 40 (mu)m in diameter) occurring at 20- to 200-(mu)m intervals. When biofilms grown on 2,4,6-TCB were shifted to a labile, nonchlorinated carbon source (Trypticase soy broth), the biofilms underwent an architectural change which included the loss of mound structures and the formation of a more homogeneous biofilm. Neutrally charged fluorescent dextrans, which upon hydration become cationic, were observed to bind to mounds, as well as to the basal cell layer, in 14-day biofilms. In contrast, polyanionic dextrans bound only to the basal cell layer, indicating that this material incorporated sites with both positive and negative charge. The results from this study indicate that nutrient composition has a significant impact on both the architecture and the physicochemistry of degradative bio...Continue Reading

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Citations

Oct 6, 2000·Microbiology·A HeydornS Molin
Dec 1, 1998·Journal of Applied Microbiology·P StoodleyH M Lappin-Scott
Apr 27, 2002·Applied and Environmental Microbiology·Bjarke B ChristensenSøren Molin
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Sep 8, 2006·Applied and Environmental Microbiology·Kristin E SearcyThomas Harter
Jun 13, 2009·FEMS Microbiology Ecology·Anne BöhmeKirsten Küsel
Oct 10, 2009·Journal of Medical Microbiology·Christian HannigAli Al-Ahmad
Feb 27, 2015·Clinical Oral Investigations·Aldo del Carpio-PerochenaMarco Hungaro Duarte
Jan 5, 2008·Biotechnology and Bioengineering·G M WolfaardtM Pilaski
Jul 23, 2019·Biomaterials Science·Haw-Young KwonYoung-Tae Chang
Sep 8, 2010·Journal of Microbiological Methods·Tom Coenye, Hans J Nelis

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