Impact of obesity control on circulating level of endothelial progenitor cells and angiogenesis in response to ischemic stimulation.

Journal of Translational Medicine
Yung-Lung ChenHon-Kan Yip

Abstract

We tested the hypothesis that obesity reduced circulating number of endothelial progenitor cells (EPCs), angiogenic ability, and blood flow in ischemic tissue that could be reversed after obesity control. 8-week-old C57BL/6J mice (n=27) were equally divided into group 1 (fed with 22-week control diet), group 2 (22-week high fat diet), and group 3 (14-week high fat diet, followed by 8-week control diet). Critical limb ischemia (CLI) was induced at week 20 in groups 2 and 3. The animals were sacrificed at the end of 22 weeks. Heart weight, body weight, abdominal fat weight, serum total cholesterol level, and fasting blood sugar were highest in group 2 (all p<0.001). The numbers of circulating EPCs (C-kit/CD31+, Sca-1/KDR + and CXCR4/CD34+) were lower in groups 1 and 2 than in group 3 at 18 h after CLI induction (p<0.03). The numbers of differentiated EPCs (C-kit/CD31+, CXCR4/CD34+ and CD133+) from adipose tissue after 14-day cultivation were also lowest in group 2 (p<0.001). Protein expressions of VCAM-1, oxidative index, Smad3, and TGF-β were higher, whereas the Smad1/5 and BMP-2, mitochondrial cytochrome-C SDF-1α and CXCR4 were lower in group 2 than in groups 1 and 3 (all p<0.02). Immunofluorescent staining of CD31+ and vWF + c...Continue Reading

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Citations

Jan 11, 2013·Journal of Translational Medicine·Wojciech BłogowskiTeresa Starzyńska
Apr 5, 2014·ISRN Obesity·Yvonne N PierpontWyatt G Payne
Nov 12, 2013·Experimental Gerontology·Gianluigi MazzoccoliManlio Vinciguerra
Mar 8, 2013·Free Radical Biology & Medicine·Richard M CubbonMark T Kearney
Oct 18, 2016·Frontiers in Physiology·Bipradas RoyHugh M Fentress
Jul 3, 2021·International Journal of Molecular Sciences·Anne-Christine PeyterCatherine Yzydorczyk

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Methods Mentioned

BETA
flow cytometry
flow cytometic
fluorescence-activated cell sorter

Software Mentioned

Labwork
SAS

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