Impact of Organic Carbon Electron Donors on Microbial Community Development under Iron- and Sulfate-Reducing Conditions

PloS One
Man Jae KwonDionysios A Antonopoulos

Abstract

Although iron- and sulfate-reducing bacteria in subsurface environments have crucial roles in biogeochemical cycling of C, Fe, and S, how specific electron donors impact the compositional structure and activity of native iron- and/or sulfate-reducing communities is largely unknown. To understand this better, we created bicarbonate-buffered batch systems in duplicate with three different electron donors (acetate, lactate, or glucose) paired with ferrihydrite and sulfate as the electron acceptors and inoculated them with subsurface sediment as the microbial inoculum. Sulfate and ferrihydrite reduction occurred simultaneously and were faster with lactate than with acetate. 16S rRNA-based sequence analysis of the communities over time revealed that Desulfotomaculum was the major driver for sulfate reduction coupled with propionate oxidation in lactate-amended incubations. The reduction of sulfate resulted in sulfide production and subsequent abiotic reduction of ferrihydrite. In contrast, glucose promoted faster reduction of ferrihydrite, but without reduction of sulfate. Interestingly, the glucose-amended incubations led to two different biogeochemical trajectories among replicate bottles that resulted in distinct coloration (whit...Continue Reading

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Citations

Feb 17, 2018·Extremophiles : Life Under Extreme Conditions·D'Arcy R Meyer-DombardCarlo A Arcilla
Jun 26, 2020·Frontiers in Microbiology·D'Arcy R Meyer-DombardJudy Malas
Dec 18, 2020·Frontiers in Microbiology·Fengfeng ZhangMikael Motelica-Heino
Mar 20, 2021·The Science of the Total Environment·Jie GaoHongchen Jiang

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Methods Mentioned

BETA
X-ray
amplicon library sequencing
454 based sequencing

Software Mentioned

fit2D
MG
FastTree
ATHENA
PyNAST
RAST
Jade
QIIME
Autobk
uclust

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