Impact of Pancreatic Rat Islet Density on Cell Survival during Hypoxia

Journal of Diabetes Research
A Rodriguez-BrotonsE Maillard

Abstract

In bioartificial pancreases (BP), the number of islets needed to restore normoglycaemia in the diabetic patient is critical. However, the confinement of a high quantity of islets in a limited space may impact islet survival, particularly in regard to the low oxygen partial pressure (PO2) in such environments. The aim of the present study was to evaluate the impact of islet number in a confined space under hypoxia on cell survival. Rat islets were seeded at three different concentrations (150, 300, and 600 Islet Equivalents (IEQ)/cm(2)) and cultured in normal atmospheric pressure (160 mmHg) as well as hypoxic conditions (15 mmHg) for 24 hours. Cell viability, function, hypoxia-induced changes in gene expression, and cytokine secretion were then assessed. Notably, hypoxia appeared to induce a decrease in viability and increasing islet density exacerbated the observed increase in cellular apoptosis as well as the loss of function. These changes were also associated with an increase in inflammatory gene transcription. Taken together, these data indicate that when a high number of islets are confined to a small space under hypoxia, cell viability and function are significantly impacted. Thus, in order to improve islet survival in th...Continue Reading

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Citations

Jun 1, 2017·Current Opinion in Organ Transplantation·Ty B DunnTimothy L Pruett
Apr 6, 2018·Pancreas·Hirotake KomatsuYoko Mullen
Jun 12, 2020·Journal of Tissue Engineering·Jordan MagissonSéverine Sigrist
May 22, 2021·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Yoshiki ImamuraYoichi Hiasa
Sep 11, 2021·Proceedings of the National Academy of Sciences of the United States of America·Kisuk YangJeffrey M Karp

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Methods Mentioned

BETA
enzyme linked immunosorbent assay
PCR
ELISA
florescence microscopy

Software Mentioned

Nis
Element
Statistics
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StatSoft

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