Impact of PEG and PEG-b-PAGE modified PLGA on nanoparticle formation, protein loading and release

International Journal of Pharmaceutics
René RietscherC Lehr

Abstract

The effect of modifying the well-established pharmaceutical polymer PLGA by different PEG-containing block-copolymers on the preparation of ovalbumin (OVA) loaded PLGA nanoparticles (NPs) was studied. The used polymers contained poly(d,l-lactic-co-glycolic acid) (PLGA), polyethylene glycol (PEG) and poly(allyl glycidyl ether) (PAGE) as building blocks. The double emulsion technique yielded spherical NPs in the size range from 170 to 220 nm (PDI<0.15) for all the differently modified polymers, allowing to directly compare protein loading of and release. PEGylation is usually believed to increase the hydrophilic character of produced particles, favoring encapsulation of hydrophilic substances. However, in this study simple PEGylation of PLGA had only a slight effect on protein release. In contrast, incorporating a PAGE block between the PEG and PLGA units, also eventually enabling active targeting introducing a reactive group, led to a significantly higher loading (+25%) and release rate (+100%), compared to PLGA and PEG-b-PLGA NPs.

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Citations

Jul 14, 2016·Chemistry, an Asian Journal·Xiaozhong Qu, Zhenzhong Yang
Sep 21, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yu WangChunming Wang
Dec 2, 2020·Materials Science & Engineering. C, Materials for Biological Applications·Akram HasanpourAmir Amani
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Oct 15, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jason Thomas DuskeyBarbara Ruozi
Jun 1, 2021·Frontiers in Chemistry·Kercia P CruzPatrícia S T Veras
Nov 26, 2021·Macromolecular Bioscience·Cristiana BertiHarm-Anton Klok

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