Impact of plants on the diversity and activity of methylotrophs in soil

Microbiome
Michael Christopher MaceyJ C Murrell

Abstract

Methanol is the second most abundant volatile organic compound in the atmosphere, with the majority produced as a metabolic by-product during plant growth. There is a large disparity between the estimated amount of methanol produced by plants and the amount which escapes to the atmosphere. This may be due to utilisation of methanol by plant-associated methanol-consuming bacteria (methylotrophs). The use of molecular probes has previously been effective in characterising the diversity of methylotrophs within the environment. Here, we developed and applied molecular probes in combination with stable isotope probing to identify the diversity, abundance and activity of methylotrophs in bulk and in plant-associated soils. Application of probes for methanol dehydrogenase genes (mxaF, xoxF, mdh2) in bulk and plant-associated soils revealed high levels of diversity of methylotrophic bacteria within the bulk soil, including Hyphomicrobium, Methylobacterium and members of the Comamonadaceae. The community of methylotrophic bacteria captured by this sequencing approach changed following plant growth. This shift in methylotrophic diversity was corroborated by identification of the active methylotrophs present in the soils by DNA stable iso...Continue Reading

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Citations

Nov 4, 2020·Environmental Pollution·Jing DingDong Zhu
Feb 24, 2021·World Journal of Microbiology & Biotechnology·Vijaya RaniRajeev Kaushik
Jun 23, 2021·Environmental Microbiome·Samuel Mm PrudenceMatthew I Hutchings
Jun 22, 2021·Chemical Communications : Chem Comm·Alexandra M Webster, Anna F A Peacock
Sep 11, 2021·Microbial Ecology·Marina L LaForgiaCassandra L Ettinger
Nov 6, 2021·Frontiers in Microbiology·Ola AlessaAkio Tani

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Datasets Mentioned

BETA
EU548063.1

Methods Mentioned

BETA
PCR
amplicon
amplicon sequencing
density gradient centrifugation
electrophoresis

Software Mentioned

QUAST
BioEdit
Megahit
MEGA7
tblastn
Metaphlan2
MetaQUAST
Trimmomatic
BLASTn
CheckM

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