Impact of pre-existing elastic matrix on TGFβ1 and HA oligomer-induced regenerative elastin repair by rat aortic smooth muscle cells.

Journal of Tissue Engineering and Regenerative Medicine
Carmen Gacchina, A Ramamurthi

Abstract

Regenerating elastic matrices lost to disease (e.g. in aneurysms) is vital to re-establishing vascular homeostasis but is challenged by the poor elastogenicity of post-neonatal cells. We previously showed that exogenous hyaluronan oligomers (HA-o) and TGFβ1 synergistically enhance tropo- and matrix elastin deposition by healthy adult rat aortic SMCs (RASMCs). Towards treating aortic aneurysms (AAs), which exhibit cause- and site-specific heterogeneity in matrix content/structure and contain proteolytically-injured SMCs, we investigated the impact of pre-existing elastic matrix degeneration on elastogenic induction of injured RASMCs. Elastin-rich RASMC layers at 21 days of culture were treated with 0.15 U/ml (PPE15) and 0.75 U/ml (PPE75) porcine pancreatic elastase to degrade the elastic matrix variably, or left uninjured (control). One set of cultures was harvested at 21 days, before and after injury, to quantify viable cell count, matrix elastin loss. Other injured cell layers were cultured to 42 days with or without factors (0.2 µg/ml HA oligomers, 1 ng/ml TGFβ1). We showed that: (a) the ability of cultures to self-repair and regenerate elastic matrices following proteolysis is limited when elastolysis is severe; (b) HA oligo...Continue Reading

References

Oct 1, 1986·Experimental and Molecular Pathology·B FarisC Franzblau
Mar 1, 1986·Journal of Ultrastructure and Molecular Structure Research·G M BressanD Volpin
Dec 1, 1985·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·M P McMahonC Franzblau
Mar 1, 1980·Analytical Biochemistry·C Labarca, K Paigen
Jul 10, 1999·The Journal of Biological Chemistry·A AspbergD Heinegård
Jun 8, 2000·Matrix Biology : Journal of the International Society for Matrix Biology·B J ReinbothE G Cleary
Dec 1, 2001·The Journal of Biological Chemistry·Zenzo IsogaiLynn Y Sakai
Jan 10, 2002·Analytical Biochemistry·Amitha H Palamakumbura, Philip C Trackman
Feb 9, 2002·Journal of Biomedical Materials Research·Anand Ramamurthi, Ivan Vesely
Sep 17, 2002·Current Opinion in Cell Biology·Thomas N Wight
Jul 31, 2003·Journal of Biomedical Materials Research. Part a·Anand Ramamurthi, Ivan Vesely
Jul 17, 2004·European Journal of Clinical Investigation·O D DefaweN Sakalihasan
Jul 17, 2004·Journal of Atherosclerosis and Thrombosis·Hiroshi WachiYoshiyuki Seyama
Mar 15, 2008·Journal of Tissue Engineering and Regenerative Medicine·Chandrasekhar R Kothapalli, Anand Ramamurthi
Nov 4, 2008·Computer Methods in Biomechanics and Biomedical Engineering·Panagiotis NeofytouMichalis Kyriakidis

❮ Previous
Next ❯

Citations

Feb 24, 2011·Tissue Engineering. Part a·Carmen E GacchinaAnand Ramamurthi
Jun 28, 2011·Tissue Engineering. Part a·Lavanya Venkataraman, Anand Ramamurthi
Jan 10, 2012·Tissue Engineering. Part B, Reviews·Chris A BashurAnand Ramamurthi
Jan 29, 2013·Drug Delivery and Translational Research·Balakrishnan SivaramanAnand Ramamurthi
Feb 20, 2016·International Journal of Biological Macromolecules·Mahadevappa HemshekharKesturu S Girish
Sep 29, 2011·Journal of Tissue Engineering and Regenerative Medicine·Chris A Bashur, Anand Ramamurthi
Jul 19, 2012·Journal of Anatomy·Tetsuaki KawaseMineko Fujimiya
Apr 17, 2014·Journal of Tissue Engineering and Regenerative Medicine·Lavanya VenkataramanAnand Ramamurthi
Apr 29, 2014·Acta Biomaterialia·Darryl K KnightKibret Mequanint
Mar 4, 2014·PloS One·Daniela Nogueira RochaAna Paula Pêgo
Dec 22, 2017·Tissue Engineering. Part a·Shataakshi DahalAnand Ramamurthi
Mar 27, 2021·Annals of Biomedical Engineering·Shataakshi DahalMargot S Damaser

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aneurysm

Aneurysms are outward distensions or bulges that occurs in a weakened wall of blood vessels. Discover the latest research on aneurysms here.

Aortic Aneurysm

An aortic aneurysm is the weakening and bulging of the blood vessel wall in the aorta. This causes dilatation of the aorta, which is usually asymptomatic but carries the risk of rupture and hemorrhage. Find the latest research on aortic aneurysms here.

Cardiac Aneurysm

Aneurysm refers to a bulge of the wall or lining of a vessel commonly occurring in the blood vessels at the base of the septum or within the aorta. In the heart, it usually arises from a patch of weakened tissue in a ventricular wall, which swells into a bubble filled with blood. Discover the latest research on cardiac aneurysm here.