Impact of processing method on donated human breast milk microRNA content

PloS One
Urszula SmyczynskaWojciech Fendler

Abstract

Pasteurization of donated human milk preserves it for storage and makes it safe for feeding, but at the expense of its composition, nutritional values and functions. Here, we aimed to investigate the impact of Holder Pasteurization (HoP) and High Pressure Processing (HPP) methods on miRNA in human milk and to evaluate impact of these changes on miRNA functions. Milk samples obtained from women in 50th day of lactation (n = 3) were subjected either to HoP, HPP or remained unpasteurized as a control. Subsequently, miRNA was isolated from whole material and exosomal fraction and sequenced with Illumina NextSeq 500. Sequencing data were processed, read counts were mapped to miRNA and analyzed both quantitatively with DESeq2 and functionally with DIANA mirPath v.3. While HPP caused statistically insignificant decrease in number of miRNA reads compared to unprocessed material, HoP led to 82-fold decrease in whole material (p = 0.0288) and 302-fold decrease in exosomes (p = 0.0021) not leaving enough reads for further analysis. Changes in composition of miRNA fraction before and after HPP indicated uneven stability of individual miRNAs under high pressure conditions, with miR-30d-5p identified as relatively stable and miR-29 family as...Continue Reading

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Citations

Oct 15, 2020·Nutrients·Elena Carrillo-LozanoCarolina Knott-Torcal
Sep 21, 2021·Frontiers in Immunology·Lina TingöMelanie Rae Simpson

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Methods Mentioned

BETA
electrophoresis

Software Mentioned

tools
Subread package
DESeq2
custom
MultiQC
featureCounts
GenePattern
UMI
TargetScan
Python

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