Impact of protein blocking on enzymatic saccharification of bagasse from sugarcane clones

Biotechnology and Bioengineering
Rafaela I S Ladeira ÁzarMichael R Ladisch

Abstract

Lignin plays an important functional and structural role in plants, but also contributes to the recalcitrance of lignocellulosic biomass to hydrolysis. This study addresses the influence of lignin in hydrolysis of sugarcane bagasse from conventional bred lines (UFV260 and UFV204) that were selected from 432 field-grown clones. In addition to higher sugar production, bagasse clone UFV204 had a small, but statistically significant, lower insoluble lignin content compared with clone UFV260 (15.5% vs, 16.6%) and also exhibited a significantly higher cellulose conversion to glucose (81.3% vs. 63.3%) at a cellulase loading of 5 (filter paper unit) FPU/g of glucan or 3 FPU/g total solids for liquid hot water pretreated bagasse (200°C, 10 min). The enzyme loading was further decreased by 50% to 2.5 FPU/g glucan and resulted in a similar glucan conversion (88.5%) for clone UFV204 when the bagasse was preincubated with bovine serum albumin at pH 4.8 and nonproductive binding of cellulase components was blocked. Comparison of Langmuir adsorption isotherms and differential adsorption of the three major cellulolytic enzyme components endoglucanase, cellobiohydrolase, and β-glucosidase help to explain differences due to lignin content.

References

Jun 19, 2007·Nature Biotechnology·Fang Chen, Richard A Dixon
Jul 11, 2007·Nature Biotechnology·Clint ChappleRick Meilan
Dec 17, 2008·Applied Biochemistry and Biotechnology·Florbela CarvalheiroFrancisco M Gírio
Sep 22, 2009·Methods in Molecular Biology·Youngmi KimMichael R Ladisch
Dec 1, 2009·Bioresource Technology·C A CardonaI C Paz
Feb 6, 2010·Nature Protocols·Gerdi Kemmer, Sandro Keller
Apr 3, 2010·Current Opinion in Plant Biology·Blake A SimmonsJohn Ralph
Nov 25, 2011·Enzyme and Microbial Technology·Eduardo XimenesMichael Ladisch
Nov 25, 2011·Enzyme and Microbial Technology·Youngmi KimMichael R Ladisch
Jan 30, 2013·Biotechnology for Biofuels·Leif J JönssonNils-Olof Nilvebrant
Jul 31, 2013·Biotechnology and Bioengineering·Youngmi KimMichael R Ladisch
May 31, 2014·The Journal of Biological Chemistry·Deanne W SammondMichael F Crowley
Aug 15, 2014·Biotechnology and Bioengineering·Ja Kyong KoMichael R Ladisch
Oct 18, 2014·Biotechnology and Bioengineering·Youngmi KimMichael R Ladisch
Dec 4, 2015·Biotechnology for Biofuels·Maira Prearo GrimaldiSandra Regina Pombeiro Sponchiado
Sep 18, 2016·Bioresource Technology·Camila FlorencioCristiane S Farinas
Dec 12, 2017·Enzyme and Microbial Technology·Rafaela I S Ladeira ÁzarValéria M Guimarães
Nov 28, 2018·Trends in Biotechnology·Antonio Carlos Dos SantosMichael R Ladisch

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Citations

Feb 7, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Rafaela I S Ladeira ÁzarDaehwan Kim
Jan 2, 2021·International Journal of Biological Macromolecules· AlokikaBijender Singh

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