Impact of Pseudomonas aeruginosa quorum sensing on cellular wound healing responses in vitro

Scandinavian Journal of Infectious Diseases
Jasper N JacobsenKaren A Krogfelt

Abstract

The virulent capacity of Pseudomonas aeruginosa can largely be ascribed to quorum sensing, i.e. the ability to evade host defence by a coordinated production and secretion of virulence factors. When P. aeruginosa is harboured in chronic wounds, a non-healing condition is often observed. In this study, we examined the in vitro cellular responses of the major cell types of re-epithelialization to supernatants of P. aeruginosa wild-type or an isogenic mutant not expressing quorum sensing-regulated virulence genes. We observed impairment of cell migration in keratinocytes (p = 0.009) and fibroblasts (p = 0.043) when supplementing medium with 20% P. aeruginosa culture supernatants. Cell proliferation was not significantly reduced, except for keratinocytes (p = 0.040). Data show compliance with in vivo observations of proliferating, non-motile epithelial cell behaviour in bacterially contaminated chronic wounds. Our findings suggest that quorum sensing may serve as an interesting target for controlling P. aeruginosa virulence in modern wound care.

References

Feb 28, 2001·Microbes and Infection·N HøibyA Kharazmi
May 11, 2002·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·B SteffensenH Larjava
Jul 26, 2003·The EMBO Journal·Morten HentzerMichael Givskov
Jul 12, 2005·The Journal of Burn Care & Rehabilitation·Abelardo MedinaEdward E Tredget
Jun 27, 2006·Plastic and Reconstructive Surgery·Thomas A MustoeOliver Kloeters
Sep 21, 2006·International Wound Journal·Kristine GjødsbølKaren A Krogfelt
Oct 5, 2006·Analytical and Bioanalytical Chemistry·Thomas Bjarnsholt, Michael Givskov
Jun 15, 2007·The International Journal of Lower Extremity Wounds·Magnus S Agren, Maria Werthén
Dec 19, 2007·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Garth A JamesPhilip S Stewart
Jan 24, 2008·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Thomas BjarnsholtMichael Givskov
Jan 24, 2008·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Gojiro NakagamiYasunori Ohta
May 23, 2008·Journal of Wound Care·R D Wolcott, D D Rhoads
May 30, 2008·Journal of Clinical Microbiology·Klaus Kirketerp-MøllerThomas Bjarnsholt
Oct 9, 2009·Journal of Clinical Microbiology·Mustafa FazliTim Tolker-Nielsen

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Citations

Dec 18, 2013·Der Hautarzt; Zeitschrift für Dermatologie, Venerologie, und verwandte Gebiete·J Dissemond
Dec 9, 2014·Journal der Deutschen Dermatologischen Gesellschaft = Journal of the German Society of Dermatology : JDDG·Finja JockenhöferJoachim Dissemond
Aug 12, 2016·Journal der Deutschen Dermatologischen Gesellschaft = Journal of the German Society of Dermatology : JDDG·Cornelia Sigrid Lissi MüllerClaudia Pföhler
Aug 12, 2016·Journal der Deutschen Dermatologischen Gesellschaft = Journal of the German Society of Dermatology : JDDG·Cornelia Sigrid Lissi MüllerClaudia Pföhler
Nov 9, 2018·The Journal of Pharmacy and Pharmacology·Michaela EisenhardtFrank Runkel
Oct 16, 2018·Environmental Microbiology·Yiying LiuTom Defoirdt
May 15, 2016·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Manon RuffinEmmanuelle Brochiero
Oct 4, 2015·FEMS Microbiology Reviews·Catherine GrandclémentDenis D Faure
Jun 20, 2019·Frontiers in Cellular and Infection Microbiology·Manon Ruffin, Emmanuelle Brochiero
Jun 29, 2021·Virulence·Charles D MorinGee W Lau

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