Impact of recombinant adenovirus serotype 35 priming versus boosting of a Plasmodium falciparum protein: characterization of T- and B-cell responses to liver-stage antigen 1.

Infection and Immunity
Ariane RodríguezKatarina Radosević

Abstract

Prime-boost vaccination regimens with heterologous antigen delivery systems have indicated that redirection of the immune response is feasible. We showed earlier that T-cell responses to circumsporozoite (CS) protein improved significantly when the protein is primed with recombinant adenovirus serotype 35 coding for CS (rAd35.CS). The current study was designed to answer the question whether such an effect can be extended to liver-stage antigens (LSA) of Plasmodium falciparum such as LSA-1. Studies with mice have demonstrated that the LSA-1 protein induces strong antibody response but a weak T-cell immunity. We first identified T-cell epitopes in LSA-1 by use of intracellular gamma interferon (IFN-gamma) staining and confirmed these epitopes by means of enzyme-linked immunospot assay and pentamer staining. We show that a single immunization with rAd35.LSA-1 induced a strong antigen-specific IFN-gamma CD8(+) T-cell response but no measurable antibody response. In contrast, vaccinations with the adjuvanted recombinant LSA-1 protein induced remarkably low cellular responses but strong antibody responses. Finally, both priming and boosting of the adjuvanted protein by rAd35 resulted in enhanced T-cell responses without impairing th...Continue Reading

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Citations

Dec 17, 2009·Expert Reviews in Molecular Medicine·Ruobing WangStefan H I Kappe
Aug 21, 2009·Parasite Immunology·K J Limbach, T L Richie
Jul 15, 2015·Current Opinion in Immunology·Alberto Moreno, Chester Joyner
May 11, 2016·Expert Review of Vaccines·Karen A MartinsJens H Kuhn
Apr 29, 2009·Expert Review of Vaccines·Katarina RadosevićJaap Goudsmit
Nov 28, 2012·Expert Review of Vaccines·Patrick E DuffyCharles Anderson
Aug 31, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Monica Cabrera-MoraAlberto Moreno

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