PMID: 26320498Sep 1, 2015Paper

Impact of RGD Peptide Tethering to IL24/mda-7 (Melanoma Differentiation Associated Gene-7) on Apoptosis Induction in Hepatocellular Carcinoma Cells

Asian Pacific Journal of Cancer Prevention : APJCP
Samaneh BinaSeyed Younes Hosseini

Abstract

Melanoma differentiation-associated gene-7 (MDA-7)/interleukin-24 (IL-24), a unique tumor suppressor gene, has killing activity in a broad spectrum of cancer cells. Herein, plasmids producing mda-7 proteins fused to different RGD peptides (full RGD4C and shortened RGD, tRGD) were evaluated for apoptosis induction with a hepatocellular carcinoma cell line, Hep-G2. The study aim was to improve the apoptosis potency of mda-7 by tethering to RGD peptides. Three plasmids including mda-7, mda-7-RGD and mda-7-tRGD genes beside a control vector were transfected into Hep-G2 cells. After 72 hours incubation, cell viability was evaluated by MTT assay. In addition, the rate of apoptosis was analyzed by flow cytometry using PI/annexin staining. To detect early events in apoptosis, 18 hours after transfection, expression of the BAX gene was quantified by real time PCR. Modeling of proteins was also performed to extrapolate possible consequences of RGD modification on their structures and subsequent attachment to receptors. In MTT assays, while all mda-7 forms showed measurable inhibition of proliferation, unmodified mda-7 protein exhibited most significant effect compared to control plasmid (P<0.001). Again, flow cytometry analysis showed a ...Continue Reading

References

Aug 20, 1996·Proceedings of the National Academy of Sciences of the United States of America·H JiangP B Fisher
Jun 27, 2000·Bioinformatics·L J McGuffinD T Jones
Jun 11, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Eva G CaudellElizabeth A Grimm
Jul 13, 2002·Proceedings of the National Academy of Sciences of the United States of America·Devanand SarkarPaul B Fisher
Jun 18, 2003·Journal of Cellular Physiology·Moira SauanePaul B Fisher
Jun 26, 2003·Nucleic Acids Research·Torsten SchwedeManuel C Peitsch
Aug 19, 2003·Biomaterials·Ulrich HerselHorst Kessler
Jul 6, 2004·Protein Engineering, Design & Selection : PEDS·Peter HöligRoland E Kontermann
Nov 27, 2004·Molecular Therapy : the Journal of the American Society of Gene Therapy·Sunil ChadaSuhendan Ekmekcioglu
Jul 21, 2005·Technology in Cancer Research & Treatment·Masato Yamamoto, David T Curiel
Nov 17, 2005·Cancer Research·Paul B Fisher
Feb 9, 2006·Pharmacology & Therapeutics·Pankaj GuptaPaul B Fisher
Nov 11, 2006·Cancer Gene Therapy·M Bazan-PeregrinoA L Harris
May 5, 2007·Nucleic Acids Research·Sitao Wu, Yang Zhang
Jan 25, 2008·BMC Bioinformatics·Yang Zhang
Apr 5, 2008·Molecular Therapy : the Journal of the American Society of Gene Therapy·Ryan CraigShulin Li
Jan 18, 2012·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Dong-Sheng PeiJun-Nian Zheng
Jul 18, 2012·Proceedings of the National Academy of Sciences of the United States of America·Naomi J LogsdonMark R Walter
Sep 18, 2012·Cytokine & Growth Factor Reviews·Erin L WhitakerPenelope J Duerksen-Hughes
May 1, 2008·Molecular Therapy : the Journal of the American Society of Gene Therapy·Ryan CraigShulin Li

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