Impact of Side Chain Polarity on Non-Stoichiometric Nano-Hydroxyapatite Surface Functionalization with Amino Acids

Scientific Reports
Patricia Comeau, Thomas Willett

Abstract

In this study the affinity of three amino acids for the surface of non-stoichiometric hydroxyapatite nanoparticles (ns-nHA) was investigated under different reaction conditions. The amino acids investigated were chosen based on their differences in side chain polarity and potential impact on this surface affinity. While calcium pre-saturation of the calcium-deficient ns-nHA was not found to improve attachment of any of the amino acids studied, the polarity and fraction of ionized functional side groups was found to have a significant impact on this attachment. Overall, amino acid attachment to ns-nHA was not solely reliant on carboxyl groups. In fact, it seems that amine groups also notably interacted with the negative ns-nHA surface and increased the degree of surface binding achieved. As a result, glycine and lysine had greater attachment to ns-nHA than aspartic acid under the reaction conditions studied. Lastly, our results suggest that a layer of each amino acid forms at the surface of ns-nHA, with aspartic acid attachment the most stable and its surface coverage the least of the three amino acids studied.

References

Oct 26, 2000·Journal of Colloid and Interface Science·S Koutsopoulos, E Dalas
May 15, 2001·Journal of Colloid and Interface Science·Gamal M. S. El Shafei, Nabawaya A. Moussa
Jun 13, 2001·Journal of Colloid and Interface Science·Nikos SpanosPetros G. Koutsoukos
Jul 31, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Haihua PanRuikang Tang
Oct 30, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Kevin S JackMatt Trau
Feb 22, 2012·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·Albert RimolaPiero Ugliengo
Sep 26, 2012·Journal of Biomedical Materials Research. Part a·Wing-Hin LeeRamin Rohanizadeh
Dec 28, 2012·Journal of the Royal Society, Interface·M Tavafoghi JahromiM Cerruti
Aug 6, 2014·Colloids and Surfaces. B, Biointerfaces·Wing-Hin LeeRamin Rohanizadeh
Oct 28, 2014·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Luis J Del ValleCarlos Alemán

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Citations

Nov 19, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Sergey V Dorozhkin
Dec 2, 2020·Materials Science & Engineering. C, Materials for Biological Applications·Dibakar MondalThomas L Willett
Feb 7, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Neelam Chauhan, Yashveer Singh
May 16, 2020·Materials Science & Engineering. C, Materials for Biological Applications·P A Comeau, T L Willett
Oct 30, 2021·Environmental Science & Technology·Regiane NatumiElisabeth M-L Janssen

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Methods Mentioned

BETA
scanning electron microscopy

Software Mentioned

DMFit
ZetaQ
SoftMax Pro
SPSS® Statistics

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