Impact of solid surface hydrophobicity and micrococcal nuclease production on Staphylococcus aureus Newman biofilms.

Scientific Reports
Abigail M ForsonJelmer Sjollema

Abstract

Staphylococcus aureus is commonly associated with biofilm-related infections and contributes to the large financial loss that accompany nosocomial infections. The micrococcal nuclease Nuc1 enzyme limits biofilm formation via cleavage of eDNA, a structural component of the biofilm matrix. Solid surface hydrophobicity influences bacterial adhesion forces and may as well influence eDNA production. Therefore, it is hypothesized that the impact of Nuc1 activity is dependent on surface characteristics of solid surfaces. For this reason, this study investigated the influence of solid surface hydrophobicity on S. aureus Newman biofilms where Nuc1 is constitutively produced. To this end, biofilms of both a wild-type and a nuc1 knockout mutant strain, grown on glass, salinized glass and Pluronic F-127-coated silanized glass were analysed. Results indicated that biofilms can grow in the presence of Nuc1 activity. Also, Nuc1 and solid surface hydrophobicity significantly affected the biofilm 3D-architecture. In particular, biofilm densities of the wild-type strain on hydrophilic surfaces appeared higher than of the mutant nuc1 knockout strain. Since virulence is related to bacterial cell densities, this suggests that the virulence of S. au...Continue Reading

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Citations

Apr 14, 2021·Environmental Science and Pollution Research International·Eman A MahmoudShaimaa A M Aboeldahb
Jun 15, 2021·Frontiers in Microbiology·Donald C HallJarosław E Król
Jul 17, 2021·The Journal of Biological Chemistry·Zahra NajarzadehDaniel E Otzen
Jul 30, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Arash MazinaniDusan Losic
Aug 26, 2021·Biochemistry·Ajay Singh SawnerSamir K Maji

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Methods Mentioned

BETA
Fluorescence

Software Mentioned

GraphPad
GraphPad Prism
MATLAB

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