Impact of Spaceflight and Artificial Gravity on the Mouse Retina: Biochemical and Proteomic Analysis

International Journal of Molecular Sciences
Xiao Wen MaoMichael D Delp

Abstract

Astronauts are reported to have experienced some impairment in visual acuity during their mission on the International Space Station (ISS) and after they returned to Earth. There is emerging evidence that changes in vision may involve alterations in ocular structure and function. To investigate possible mechanisms, changes in protein expression profiles and oxidative stress-associated apoptosis were examined in mouse ocular tissue after spaceflight. Nine-week-old male C57BL/6 mice (n = 12) were launched from the Kennedy Space Center on a SpaceX rocket to the ISS for a 35-day mission. The animals were housed in the mouse Habitat Cage Unit (HCU) in the Japan Aerospace Exploration Agency (JAXA) "Kibo" facility on the ISS. The flight mice lived either under an ambient microgravity condition (µg) or in a centrifugal habitat unit that produced 1 g artificial gravity (µg + 1 g). Habitat control (HC) and vivarium control mice lived on Earth in HCUs or normal vivarium cages, respectively. Quantitative assessment of ocular tissue demonstrated that the µg group induced significant apoptosis in the retina vascular endothelial cells compared to all other groups (p < 0.05) that was 64% greater than that in the HC group. Proteomic analysis sh...Continue Reading

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Citations

Sep 17, 2019·Clinical and Translational Science·Catherine K YeungEdward J Kelly
Apr 2, 2020·International Journal of Molecular Sciences·Chiara De CesariDebora Angeloni
Apr 16, 2020·Journal of Neurophysiology·Gilles R ClémentWilliam H Paloski
Jun 23, 2019·International Journal of Molecular Sciences·Victoria GulimovaSergey Saveliev
Sep 26, 2020·Frontiers in Physiology·Nastassia NavasiolavaMarc-Antoine Custaud
Nov 6, 2020·Neuroscience and Biobehavioral Reviews·Richard Boyle
Jan 23, 2021·Genes·Miroslav PribylIva Kubikova
Dec 16, 2020·International Journal of Molecular Sciences·Binod PrasadMarcus Krüger
Jul 20, 2021·IScience·Keisuke YoshidaShunsuke Ishii

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Methods Mentioned

BETA
ISS
dissections

Software Mentioned

Perseus
R studio
pheatmap R
ImageJ
Systat
Sigma Plot
Andromeda
MaxQuant

Related Concepts

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis