Impact of steroid hormones E2 and P on the NLRP3/ASC/Casp1 axis in primary mouse astroglia and BV-2 cells after in vitro hypoxia

The Journal of Steroid Biochemistry and Molecular Biology
Alexander SlowikCordian Beyer

Abstract

Clinical and animal model studies have demonstrated the neuroprotective and anti-inflammatory effects of 17beta-estradiol (E2) and progesterone (P) in different disease models of the central nervous system (CNS) including ischemic stroke. Inflammasomes are involved in the interleukin-1 beta (IL1beta) maturation, in particular, NLRP3, the adaptor protein apoptosis-associated speck-like protein containing a CARD (ASC) and the active caspase-1 (Casp1) form. Recently, we showed that administration of E2 or P selectively regulated these components after experimental ischemic stroke in rats. Therefore, we investigated the impact of E2 and P on the NLRP3/ASC/Casp1 axis in the murine microglia-like cell line BV-2 cells and primary astrocytes after short-term in vitro hypoxia. The inflammatory cytokine IL1beta but not IL18 was increased after short-term hypoxia in astroglia and BV-2 cells. The same applied to NLPR3 and ASC. Casp1 activity was also elevated in astroglia and BV-2 cells after hypoxia. The administration of E2 or P selectively dampened IL1beta, ASC and NLRP3 expression mainly in BV-2 cells. Both steroid hormones failed to reduce Casp1 activity after hypoxia. We conclude that E2- and P-mediated anti-inflammatory mechanisms o...Continue Reading

Citations

May 30, 2020·International Journal of Molecular Sciences·Claudia Espinosa-GarciaDonald G Stein
Jun 10, 2020·The Journal of Pharmacology and Experimental Therapeutics·Maricedes Acosta-Martinez
Mar 16, 2019·International Journal of Molecular Sciences·Fatemeh Ranjbar TaklimieAdib Zendedel
Jul 11, 2020·International Journal of Molecular Sciences·Pardes HabibAlexander Slowik
Dec 1, 2020·Frontiers in Neuroscience·Xu YanXinwen Zhang
Jun 1, 2021·Journal of Leukocyte Biology·Sahana NagarajaSannula Kesavardhana

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