Impact of storage conditions on metabolite profiles of sputum samples from persons with cystic fibrosis

Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society
Jiangchao ZhaoJohn J LiPuma

Abstract

Although recent studies have begun to elucidate how airway microbial community structure relates to lung disease in cystic fibrosis (CF), microbial community activity and the host's response to changes in this activity are poorly understood. Metabolomic profiling provides a means to investigate microbial activity and human cell activity within diseased airways. However, variables in sample storage and shipping likely affect downstream analyses and standards for sample handling are lacking. We assessed the impact of sample storage conditions on liquid chromatography mass spectrometry analysis of CF sputum samples. Significant changes in global metabolomic profiles occurred in samples stored at room temperature or at 4°C for longer than one day. Untargeted metabolomic profiles were stable in sputum samples stored at -20°C or -80°C for at least 28 days. Quorum sensing molecules and phenazines, both considered important to the in vivo activity of Pseudomonas during airway infection, were detected after sample storage at room temperature for five days. Sputum samples can be stored at -20°C or -80°C for weeks with minimal effect on global metabolomic profiles. This observation provides guidance in designing metabolomic studies that h...Continue Reading

References

Feb 18, 2006·International Journal of Medical Microbiology : IJMM·Stephen P DiggleMiguel Cámara
Sep 12, 2006·Analytical Biochemistry·Elizabeth Kimball, Joshua D Rabinowitz
Feb 25, 2009·Analytical Chemistry·Jörg Martin BüscherNicola Zamboni
Mar 28, 2012·Proceedings of the National Academy of Sciences of the United States of America·Jiangchao ZhaoJohn J LiPuma
May 15, 2012·Free Radical Biology & Medicine·Jun YangBruce D Hammock
Jul 5, 2012·Annals of the New York Academy of Sciences·Jason P EiserichCarroll E Cross
Aug 7, 2012·American Journal of Respiratory Cell and Molecular Biology·Ryan C HunterDianne K Newman
May 7, 2013·PloS One·Edith T ZemanickTheresa A Laguna
Jun 28, 2013·Annals of the American Thoracic Society·Lisa A CarmodyJohn J LiPuma
Dec 25, 2013·Metabolomics : Official Journal of the Metabolomic Society·Justyna KozlowskaGeraint B Rogers
Jul 30, 2014·Annals of the American Thoracic Society·Perry S BrownLucas R Hoffman

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Citations

Nov 4, 2015·Annual Review of Physiology·Robert P DicksonGary B Huffnagle
Mar 15, 2016·Frontiers in Immunology·Kathleen A StringerPaige Lacy
Sep 4, 2015·Pediatric Pulmonology·Lindsay J CaverlyJohn J LiPuma
Nov 7, 2015·Molecular and Cellular Pediatrics·Marianne S Muhlebach, Wei Sha
May 15, 2020·Nature Protocols·Allegra T AronPieter C Dorrestein

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