Impact of surgery on nitric oxide in rats: evidence for activation of inducible nitric oxide synthase

Kidney International
G LosonczyC Baylis

Abstract

We investigated the effect of euvolemic surgical preparation, on chemical indices of activity of the nitric oxide (NO) system, in anesthetized, acutely prepared rats. The urinary excretion of NO2+NO3 (UNOXV) and cGMP (UcGMPV) increased progressively during the experiment. Pretreatment with aminoguanidine or dexamethasone, inhibitors of inducible NO synthase (iNOS), prevented the increase in UNOXV and UcGMPV but had no impact on mean arterial pressure (BP), renal vascular resistance (RVR) or GFR. Since these variables did not change in the conscious rat, the increased UNOXV results from some aspect of the acute surgical preparation. When acutely prepared rats received L-NAME, a non-specific NOS inhibitor, BP and RVR increased but paradoxical increases in UNOXV and UcGMPV were also seen. Nonselective NOS inhibition (+L-NAME) was fatal in 50% of acutely prepared rats, causing cardiac contracture. The same dose of L-NAME produced no deaths in either conscious chronically catheterized rats or in acutely prepared rats, previously subjected to sterile surgery and acute L-NAME in the conscious state. These data indicate that acute, nonsterile surgery induces expression of iNOS, but that the additional NO generated has no obvious cardio...Continue Reading

References

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Citations

Jun 28, 2000·Cardiovascular Research·G LosonczyC Baylis
May 28, 2005·The Journal of Trauma·Kerstin MetcalfBjorn Lisander
May 8, 2001·Clinical and Experimental Hypertension : CHE·I O Dedeoglu, J E Springate
Aug 17, 2001·European Journal of Gastroenterology & Hepatology·T C SchmandraE W Hanisch
Sep 18, 2002·The Journal of Pharmacology and Experimental Therapeutics·Thomas E N JonassenNiels V Olsen
Apr 18, 2002·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Alp Sener, Francine G Smith
Apr 6, 2000·American Journal of Physiology. Heart and Circulatory Physiology·M L EntmanL H Michael
Apr 12, 2001·American Journal of Physiology. Heart and Circulatory Physiology·A Sener, F G Smith
Aug 15, 2001·American Journal of Physiology. Renal Physiology·M LiangF G Knox
Mar 10, 1999·The American Journal of Physiology·T KristonG Losonczy
Jun 19, 2001·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·M KakokiD L Mattson
Jun 18, 2005·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·David L Mattson, Carla J Meister
Jun 11, 1999·The American Journal of Physiology·J M ValdivielsoJ M López-Novoa
Sep 26, 2017·Journal of Neurosurgical Sciences·Daniel BabulaJacek Kurzepa
Jan 5, 2000·Journal of the American Society of Nephrology : JASN·Roland VeelkenR Bernd Sterzel

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