Impact of temperature dependent sampling procedures in proteomics and peptidomics--a characterization of the liver and pancreas post mortem degradome.

Molecular & Cellular Proteomics : MCP
Birger ScholzPeter James

Abstract

Little is known about the nature of post mortem degradation of proteins and peptides on a global level, the so-called degradome. This is especially true for nonneural tissues. Degradome properties in relation to sampling procedures on different tissues are of great importance for the studies of, for instance, post translational modifications and/or the establishment of clinical biobanks. Here, snap freezing of fresh (<2 min post mortem time) mouse liver and pancreas tissue is compared with rapid heat stabilization with regard to effects on the proteome (using two-dimensional differential in-gel electrophoresis) and peptidome (using label free liquid chromatography). We report several proteins and peptides that exhibit heightened degradation sensitivity, for instance superoxide dismutase in liver, and peptidyl-prolyl cis-trans isomerase and insulin C-peptides in pancreas. Tissue sampling based on snap freezing produces a greater amount of degradation products and lower levels of endogenous peptides than rapid heat stabilization. We also demonstrate that solely snap freezing related degradation can be attenuated by subsequent heat stabilization. We conclude that tissue sampling involving a rapid heat stabilization step is prefera...Continue Reading

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Citations

Sep 11, 2010·Journal of the American Society for Mass Spectrometry·Alexandra van RemoortereLiam A McDonnell
Jul 27, 2011·Biochemistry·Arthur D Tinoco, Alan Saghatelian
Feb 6, 2013·Molecular & Cellular Proteomics : MCP·Karl SköldBirger Scholz
Feb 14, 2013·Biochimica Et Biophysica Acta·Julia S GelmanLloyd D Fricker
Jun 30, 2012·Journal of Proteomics·Emrys A JonesLiam A McDonnell
Nov 25, 2014·Analytical and Bioanalytical Chemistry·Sravani MusunuriGanna Shevchenko
Sep 30, 2016·PloS One·Sayani DasguptaLloyd D Fricker
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Dec 10, 2013·Journal of Proteome Research·Liam A McDonnellGarry L Corthals
Mar 20, 2012·Journal of Proteome Research·Xiaozhe ZhangGregor Rainer
Feb 16, 2013·Journal of Proteome Research·Oskar KarlssonEva B Brittebo

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