Impact of the glycostructure of amphiphilic membrane components on the function of the outer membrane of Gram-negative bacteria as a matrix for incorporated channels and a target for antimicrobial peptides or proteins

European Journal of Cell Biology
Thomas Gutsmann, U Seydel

Abstract

The architecture of the lipid matrix of Gram-negative bacteria is extremely asymmetric with respect to its lipid distribution: Whereas the inner leaflet is composed of a phospholipid mixture, the outer leaflet is built up by glycolipids. For most Gram-negative species, these glycolipids are lipopolysaccharides, for a few species, however, glycosphingolipids. We describe here our experimental approach and results thereof to get answers on the function of the asymmetric architecture and, in particular of the glycostructure of the outer leaflet (i) for the incorporation of porin channels into the bilayer and their function inside the membrane, (ii) the role of the glycolipid surface in the activation of the complement system, and (iii) the formation of transient lesions or stable pores by the interaction of antimicrobial peptides, e.g. polymyxin B, the bactericidal/permeability-increasing protein BPI, and cathelicidins. Furthermore, we investigated the influence of the glycocomponent on basic biophysical characteristics of glycolipid-containing membranes such as their electric properties and the lateral organization of the glycolipids in the membrane. To this end we established and applied a number of various reconstitution system...Continue Reading

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Citations

May 5, 2012·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·David B AlexanderHiroyuki Tsuda
Oct 30, 2014·Environmental Science & Technology·Jenia GutmanSharon L Walker
Jul 13, 2014·Biochimica Et Biophysica Acta·Wilmar CorreaLena Heinbockel
Jun 8, 2014·Colloids and Surfaces. B, Biointerfaces·Magdalena WytrwalMariusz Kepczynski
Jan 18, 2014·Biomacromolecules·Naoki Yamamoto, Atsuo Tamura
Mar 16, 2018·Clinical Microbiology Reviews·Ronald DomalaonFrank Schweizer
Sep 21, 2015·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Sudeep GoswamiAiyagari Ramesh
Nov 16, 2018·Journal of Colloid and Interface Science·Daniela CiumacJian Ren Lu

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