Impact of thyroid hormone deficiency on the developing CNS: cerebellar glial and neuronal protein expression in rat neonates exposed to antithyroid drug propylthiouracil

The Cerebellum
Gui-Hua LiE M Sajdel-Sulkowska

Abstract

The developing rat cerebellum is vulnerable to thyroid hormone (TH) deficiency. The present study addresses the molecular mechanisms involved in this response. Specifically, the study focuses on the expression of selected cerebellar proteins that are known to be directly [protein expressing 3-fucosyl-N-acetyl-lactosamine antigen (CD15), neuronal cell adhesion molecule (L1)] or indirectly [glial fibrillary acidic protein (GFAP)], involved in glial-neuronal interactions and thus regulation of cell proliferation and granule cell migration. Cerebellar mass, structure, and protein expression in rat neonates exposed to antithyroid drug propylthiouracil (PTU) from the embryonic day (E) 16 to postnatal day (P) 21 were compared against rat neonates that received replacement of thyroxin (T4) starting on day P1 or untreated controls. Cerebellar proteins were analyzed by quantitative Western blots. PTU-treated rats lagged in growth and showed reduction in cerebellar mass and alterations in cerebellar structure on P15. Daily treatment of neonates with T4 restored normal cerebellum-to-body-mass ratio, cerebellar structure, and cerebellar protein expression. Densitometric analysis of Western blots revealed altered expression of selected prote...Continue Reading

Citations

Oct 25, 2007·Thyroid : Official Journal of the American Thyroid Association·R Thomas Zoeller
Nov 23, 2006·Annals of the New York Academy of Sciences·R Thomas Zoeller
Jun 20, 2013·Thyroid : Official Journal of the American Thyroid Association·Eduardo A AlbornozClaudia A Riedel
Mar 19, 2011·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·R G Ahmed
Aug 8, 2018·Toxicological Sciences : an Official Journal of the Society of Toxicology·Katherine L O'ShaughnessyMary E Gilbert
Jul 3, 2020·Endocrinology·Mary E GilbertMarta Axelstad

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