Impact of tobacco smoke on interleukin-16 protein in human airways, lymphoid tissue and T lymphocytes

Clinical and Experimental Immunology
A AnderssonAnders Lindén

Abstract

CD4(+) and CD8(+) lymphocytes are mobilized in severe chronic obstructive pulmonary disease (COPD) and the CD8(+) cytokine interleukin (IL)-16 is believed to be important in regulating the recruitment and activity of CD4(+) lymphocytes. In the current study, we examined whether tobacco smoke exerts an impact not only on IL-16 in the lower airways but also in CD4(+) or CD8(+) lymphocytes or in lymphoid tissue. The concentration of IL-16 protein was measured by enzyme-linked immunosorbent assay (ELISA) in concentrated bronchoalveolar lavage fluid (BALF) collected from 33 smokers with chronic bronchitis (CB), eight asymptomatic smokers (AS) and seven healthy never-smokers (NS). The concentrations of IL-16 and soluble IL-2 receptor alpha (sIL-2Ralpha) protein were also measured in conditioned medium from human blood CD4(+) and CD8(+) lymphocytes stimulated with tobacco smoke extract (TSE) in vitro. IL-16 mRNA was assessed in vitro as well, using reverse transcription-polymerase chain reaction (RT-PCR). Finally, the intracellular immunoreactivity for IL-16 protein (IL-16IR) was assessed in six matched pairs of palatine tonsils from smokers and non-smokers. BALF IL-16 was higher in CB and AS than in NS. TSE substantially increased th...Continue Reading

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Citations

Oct 22, 2013·Omics : a Journal of Integrative Biology·Russell P BowlerKaterina J Kechris
Jan 2, 2014·Toxicology and Applied Pharmacology·Yuan Xu, Lars-Olaf Cardell
Feb 26, 2015·Human Vaccines & Immunotherapeutics·Jian DuErping Xu
Mar 7, 2014·Journal of Receptor and Signal Transduction Research·Hiroyuki AmanoYoshitoshi Kasuya
Aug 14, 2008·BMC Immunology·Miljana MomcilovićMarija Mostarica-Stojković
Dec 20, 2011·Journal of Translational Medicine·Cailin M WilkeWeiping Zou
Mar 31, 2015·PloS One·Chantal DonovanJane E Bourke
Aug 14, 2008·PloS One·Johann PötzlAnja Lechner
Jun 24, 2016·Annals of Intensive Care·Cong-Juan LuoXiang-Mei Chen
Nov 21, 2007·Brain, Behavior, and Immunity·Junping XinKathryn J Jones
May 5, 2009·The Journal of Allergy and Clinical Immunology·Jennifer LoutenRene de Waal Malefyt
Mar 24, 2010·Journal of Immunotoxicology·Santosh YadavHe Wang
Nov 3, 2010·American Journal of Physiology. Lung Cellular and Molecular Physiology·Anders AnderssonAnders Lindén
Jun 23, 2019·Toxicological Sciences : an Official Journal of the Society of Toxicology·Ayman AlhamdowKarin Broberg
Apr 14, 2020·International Journal of Chronic Obstructive Pulmonary Disease·MeiLan K Han

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