Impact of verification media and resuscitation on accuracy of the membrane filter total coliform enumeration technique.

Applied and Environmental Microbiology
T M EvansM W LeChevallier

Abstract

Verification of membrane filter total coliform colonies was compared in lauryl tryptose broth, and m-LAC broth primary media and brilliant green-lactose-bile broth and EC broth secondary media. Verification in m-LAC broth yielded the greatest number of aerogenic isolates for both untreated surface water and drinking water samples. Verification in brilliant green-lactose-bile broth increased the number of false-negative reactions. At least 90% of the isolates aerogenic in primary verification media and anaerogenic in brilliant green-lactose-bile broth were representative of the coliform genera. The addition of a resuscitation step in the membrane filter technique did not yield greater numbers of verified coliforms per sample. Verification of both typical and atypical colonies in m-LAC broth resulted in a 10-fold increase in coliform numbers from untreated surface water. With drinking water, verification of both colony types resulted in an increase from less than 1 coliform per 100 ml to greater than 1/100 ml. A single-step verification in m-LAC broth is proposed as a more rapid and sensitive coliform verification procedure than the standard technique.

References

Apr 1, 1977·Canadian Journal of Microbiology·J A Clark, J E Pagel
May 1, 1976·Canadian Journal of Microbiology·B J Dutkaand, S E Tobin
Oct 1, 1976·Applied and Environmental Microbiology·C V DavenportR C Gordon
Nov 1, 1970·Applied Microbiology·L J SchiffJ V Mayeux
Jan 1, 1981·Applied and Environmental Microbiology·T M EvansM W LeChevallier

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Citations

Mar 28, 2002·Applied and Environmental Microbiology·A LeclercqP Baylac
Sep 1, 1989·Applied and Environmental Microbiology·J M BifulcoG K Bissonnette
Feb 1, 1983·Applied and Environmental Microbiology·M W LeChevallierG A McFeters
Aug 1, 1982·Applied and Environmental Microbiology·S C Hsu, T J Williams
Oct 1, 1982·Applied and Environmental Microbiology·J H Standridge, J J Delfino
Mar 1, 1983·Applied and Environmental Microbiology·M W LeChevallierG A McFeters
Apr 1, 1985·Applied and Environmental Microbiology·J P Petzel, P A Hartman
Jan 1, 1982·Applied and Environmental Microbiology·G A McFetersM W LeChevallier

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