Impacts of Sub-lethal DDT Exposures on microRNA and Putative Target Transcript Expression in DDT Resistant and Susceptible Drosophila melanogaster Strains

Frontiers in Genetics
Keon Mook SeongBarry R Pittendrigh

Abstract

Ten constitutively differentially expressed miRNAs were previously described between DDT-resistant 91-R and -susceptible control Drosophila melanogaster strains, and among their predicted target genes were those associated with metabolic DDT resistance mechanisms. The present study evaluated the inducibility of miRNA expression and putative downstream regulation of cytochrome P450s in response to DDT exposure in a time-dependent manner in 91-R and the susceptible Canton-S strain. Specifically, RT-qPCR analysis showed that DDT exposures led to the significant down-regulation (repression) of miR-310-3p, miR-311-3p, miR-312-3p, miR-313-3p, and miR-92a-3p levels in Canton-S. This is contrasted with the lack of significant changes in 91-R at most time-points following DDT exposure. The levels of expression among miRNAs exhibited opposite expression patterns compared to their corresponding putative target cytochrome P450s at the same time points after DDT exposure. Collectively, results from this study suggest that miR-310-3p, miR-311-3p, miR-312-3p, miR-313-3p, and miR-92a-3p might have a potential role in the control of DDT detoxification through the post-transcriptional regulation of target cytochrome P450s in Canton-S. Conversely...Continue Reading

References

Feb 17, 1999·Annual Review of Entomology·R Feyereisen
Jan 8, 2004·Genome Biology·Anton J EnrightDebora S Marks
May 1, 2004·Proceedings of the National Academy of Sciences of the United States of America·J H F PedraBarry R Pittendrigh
Sep 17, 2004·Nature·Victor Ambros
Jan 10, 2006·Methods in Enzymology·Chen-Pei D Tu, Bünyamin Akgül
Jul 18, 2006·Nucleic Acids Research·Jan Krüger, Marc Rehmsmeier
Aug 24, 2006·Annual Review of Entomology·Xianchun LiMay R Berenbaum
Nov 14, 2006·Insect Biochemistry and Molecular Biology·Lee WilloughbyPhillip J Daborn
Jun 13, 2008·Nature Protocols·Thomas D Schmittgen, Kenneth J Livak
May 2, 2009·Nucleic Acids Research·M MaragkakisA G Hatzigeorgiou
Jun 29, 2010·Pest Management Science·Robert T JonesJean M H van den Elsen
Nov 1, 1989·Trends in Ecology & Evolution·J Mallet
Aug 29, 2012·Proceedings of the National Academy of Sciences of the United States of America·Yue QiuRené Feyereisen
Dec 5, 2012·Journal of Nucleic Acids·Juan Wang, Qinghua Cui
May 25, 2013·Nucleic Acids Research·Claudia Coronnello, Panayiotis V Benos
Jun 12, 2013·Environmental Science & Technology·Lu WangEvan P Gallagher
Aug 3, 2013·Genetics·Richard H Ffrench-Constant
Oct 25, 2013·International Journal of Biological Sciences·Youhui GongNannan Liu
Dec 3, 2014·Insect Biochemistry and Molecular Biology·Shanchao HongChangliang Zhu
Jan 15, 2015·Archives of Toxicology·Hong W Yu, William C Cho
Jul 30, 2015·PloS One·Iván Sanchez-DíazEnrique Reynaud
Mar 24, 2016·Scientific Reports·Keon Mook SeongBarry R Pittendrigh
May 5, 2016·Biomedit︠s︡inskai︠a︡ khimii︠a︡·L F GulyaevaS S Nechkin
Jun 9, 2016·Environmental Toxicology and Pharmacology·Yongyong ZhouJiangfeng Guo
Oct 30, 2016·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Kai MaBo Shen
Sep 22, 2016·Parasitology Research·Mengmeng TianChangliang Zhu
Mar 28, 2017·Insect Biochemistry and Molecular Biology·Qin GuoChangliang Zhu
Apr 25, 2018·Genome Biology and Evolution·Yixin ZhaoTian Tang

❮ Previous
Next ❯

Citations

Nov 7, 2019·Pesticide Biochemistry and Physiology·Keon Mook SeongBarry R Pittendrigh
Jul 28, 2020·Pesticide Biochemistry and Physiology·Jingfei HuangBarry Robert Pittendrigh
May 7, 2021·Science Advances·Xin YangYoujun Zhang
Oct 19, 2019·Chemical Research in Toxicology·Jiakai QiaoJiangfeng Guo
Jan 29, 2022·Archives of Insect Biochemistry and Physiology·Lujuan GaoYiwen Wang

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR

Software Mentioned

RNAhybrid
miRanda
XLSTAT
DIANA
ComiR

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.