Impaired angiogenesis in the enalapril-treated neonatal rat kidney

Korean journal of pediatrics
Hyung Eun YimJoo Won Lee

Abstract

Nephrogenesis is normally accompanied by a tightly regulated and efficient vascularization. We investigated the effect of angiotensin II inhibition on angiogenesis in the developing rat kidney. Newborn rat pups were treated with enalapril (30 mg/kg/day) or vehicle (control) for 7 days after birth. Renal histological changes were checked using Hematoxylin & Eosin staining. We also investigated the intrarenal expression of vascular endothelial growth factor (VEGF)-A, VEGF receptor 1 (VEGFR1), VEGFR2, platelet-derived growth factor (PDGF)-B, and PDGF receptor-β with Western blotting and immunohistochemical staining at postnatal day 8. Expression of the endothelial cell marker CD31 was examined to determine glomerular and peritubular capillary density. Enalapril-treated rat kidneys showed disrupted tubules and vessels when compared with the control rat kidneys. In the enalapril-treated group, intrarenal VEGF-A protein expression was significantly higher, whereas VEGFR1 protein expression was lower than that in the control group (P<0.05). The expression of VEGFR2, PDGF-B, and PDGF receptor-β was not different between the 2 groups. The increased capillary CD31 expression on the western blots of enalapril-treated rat kidneys indicated...Continue Reading

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Citations

Feb 1, 2019·Pediatric Research·Kee Hwan YooYoung Sook Hong
Oct 4, 2020·Experimental and Molecular Pathology·Kee Hwan YooEun Soo Bae

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Methods Mentioned

BETA
light microscopy
electrophoresis

Software Mentioned

SPSS
Image PC

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