Nov 4, 2004

Impaired antigen presentation and effectiveness of combined active/passive immunotherapy for epithelial tumors

Journal of the National Cancer Institute
Koji MatsumotoIan H Frazer

Abstract

Although immunization with tumor antigens can eliminate many transplantable tumors in animal models, immune effector mechanisms associated with successful immunotherapy of epithelial cancers remain undefined. Skin from transgenic mice expressing the cervical cancer-associated tumor antigen human papillomavirus type 16 (HPV16) E6 or E7 proteins from a keratin 14 promoter was grafted onto syngeneic, non-transgenic mice. Skin graft rejection was measured after active immunization with HPV16 E7 and adoptive transfer of antigen-specific T cells. Cytokine secretion of lymphocytes from mice receiving skin grafts and immunotherapy was detected by enzyme-linked immunosorbent assay, and HPV16 E7-specific memory CD8+ T cells were detected by flow cytometry and ELISPOT. Skin grafts containing HPV16 E6-or E7-expressing keratinocytes were not rejected spontaneously or following immunization with E7 protein and adjuvant. Adoptive transfer of E7-specific T-cell receptor transgenic CD8+ T cells combined with immunization resulted in induction of antigen-specific interferon gamma-secreting CD8+ T cells and rejection of HPV16 E7-expressing grafts. Specific memory CD8+ T cells were generated by immunotherapy. However, a further HPV16 E7 graft was ...Continue Reading

Mentioned in this Paper

Malignant Neoplasm of Skin
KRT14 protein, human
Flow Cytometry
Interferon Gamma Measurement
Gene Expression Regulation, Neoplastic
Human Papillomavirus
T-Lymphocyte
Epithelial Ovarian Cancer
Immunologic Adjuvants
Lymphocytes as Percentage of Blood Leukocytes (Lab Test)

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