Impaired Bone Regenerative Effect of Exosomes Derived from Bone Marrow Mesenchymal Stem Cells in Type 1 Diabetes

Stem Cells Translational Medicine
Yu ZhuYi-Min Chai

Abstract

Stem cell-derived exosomes have exhibited promise for applications in tissue regeneration. However, one major problem for stem cell-derived exosome therapies is identifying appropriate source cells. In the present study, we aimed to compare the bone regenerative effect of exosomes secreted by bone marrow mesenchymal stem cells (BMSCs) derived from type 1 diabetes rats (dBMSC-exos) and exosomes secreted by BMSCs derived from normal rats (nBMSC-exos). BMSCs were isolated from rats with streptozotocin-induced diabetes and normal rats. dBMSC-exos and nBMSC-exos were isolated by an ultracentrifugation method and identified. The effects of dBMSC-exos and nBMSC-exos on the proliferation and migration of BMSCs and human umbilical vein endothelial cells (HUVECs) were investigated. The effects of exosomes on the osteogenic differentiation of BMSCs and the angiogenic activity of HUVECs were compared. Finally, a rat calvarial defect model was used to compare the effects of exosomes on bone regeneration and neovascularization in vivo. In vitro, dBMSC-exos and nBMSC-exos both enhanced the osteogenic differentiation of BMSCs and promoted the angiogenic activity of HUVECs, but nBMSC-exos had a greater effect than dBMSC-exos. Similarly, in vivo...Continue Reading

References

Aug 2, 2005·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Shinichiro KumeKensei Nagata
Mar 24, 2009·Methods in Molecular Biology·Katherine M Malinda
Apr 13, 2010·Stem Cells and Development·Christopher CramerReza Izadpanah
Oct 7, 2010·Journal of Diabetes·Subhashini Yaturu
Jan 8, 2013·International Journal of Molecular Medicine·Yan-Fang ZhaoFu-Qiang Zhang
Apr 11, 2013·Stem Cells Translational Medicine·Preeti Chhabra, Kenneth L Brayman
Nov 8, 2013·Journal of Molecular Endocrinology·Evgeny WeinbergMiron Weinreb
Sep 30, 2014·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Michael Hadjiargyrou, Regis J O'Keefe
Oct 9, 2014·Annual Review of Physiology·Stella Kourembanas
Dec 6, 2014·Journal of Dental Research·A Peer, M Khamaisi
May 24, 2016·International Journal of Molecular Sciences·Yunhao QinChangqing Zhang
Jun 2, 2016·Endocrinología y nutrición : órgano de la Sociedad Española de Endocrinología y Nutrición·Yeray Brito-CasillasAna María Wägner
Jul 20, 2016·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Jia XuGang Li
Jul 28, 2016·Stem Cells Translational Medicine·Chengcheng CuiJieli Chen
Oct 4, 2016·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Boran CaoWenbo Wang
Feb 15, 2017·Angiogenesis·Joanna FilipowskaTadeusz Niedźwiedzki
Mar 10, 2017·Orthopaedic Surgery·Priyanka GargNabil A Ebraheim
Apr 11, 2017·Acta Pharmacologica Sinica·Xin LuanDuxin Sun
Jun 26, 2017·Cells, Tissues, Organs·Ahmad OryanMohamadreza Baghaban Eslaminejad
Jun 28, 2017·Stem Cells Translational Medicine·Bi ChenYang Wang
Jul 6, 2017·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Yu MengLilach O Lerman
Jul 26, 2017·Cell Proliferation·Zi-Chen HaoShuo-Gui Xu
Nov 23, 2017·Frontiers in Bioengineering and Biotechnology·Andrea GrossoNunzia Di Maggio
Feb 13, 2018·Translational Research : the Journal of Laboratory and Clinical Medicine·Hui JingJinghao Zheng

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Citations

Aug 12, 2020·Basic & Clinical Pharmacology & Toxicology·Xiaoqin Wang, Peter Thomsen
Jan 12, 2020·Stem Cells Translational Medicine·Anthony Atala
Sep 6, 2019·Disease Markers·Christos Masaoutis, Stamatios Theocharis
Sep 19, 2019·Bioscience Trends·Jufeng XiaShunichi Arai
Jan 18, 2021·Acta Biomaterialia·Vishnu Priya Murali, Christina A Holmes
Feb 6, 2021·Stem Cells International·Bei YinLing Ye
Jun 4, 2021·Frontiers in Cell and Developmental Biology·Fanzhen HeYan Xu
Jun 8, 2021·Bone Reports·Vishnu Priya Murali, Christina A Holmes
Jun 26, 2021·Stem Cell Reviews and Reports·Kosar MalekpourMajid Ahmadi
Oct 20, 2021·ACS Applied Materials & Interfaces·Junjie ShenXianyou Zheng

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Datasets Mentioned

BETA
GM130

Methods Mentioned

BETA
electrophoresis
confocal microscopy
Assay
transmission electron microscopy
flow cytometry
light microscopy
GTPases

Software Mentioned

SPSS
ImageJ

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